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Thermo Scientific™ LanthaScreen™ TR-FRET TR beta Coactivator Assay Kit, goat

Catalog No. PV4686
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Description

The LanthaScreen™ TR-FRET TR (thyroid receptor) beta Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential TR beta ligands as agonists of ligand-dependent coactivator recruitment.

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15135:

The LanthaScreen™ TR-FRET TR (thyroid receptor) beta Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential TR beta ligands as agonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant TR beta ligand-binding domain (TR beta-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous, mix-and-read assay format.

To Assay:

When performing the LanthaScreen™ TR-FRET TR beta coactivator assay, TR beta-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound. Based on the biology of the TR beta-coactivator peptide interaction, the ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, induce a conformational change, and recruit coactivator peptide (Figure 1).

Contents and Storage:

The LanthaScreen™ TR-FRET TR beta Coactivator Assay Kit contains TR beta-LBD (GST) protein, fluorescently labeled SRC2-2 coactivator peptide, Tb-labeled anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).

TRUSTED_SUSTAINABILITY

Specifications

Conjugate Tb (Terbium)
Detection Method Fluorescence
For Use With (Equipment) Microplate Reader
Quantity 800 x 20 μL assays
Packaging 384-well Plate
Content And Storage Store in ultra-cold freezer (-68°C to -85°C).
Assay Entry Biochemical coregulator interaction
Readout End Point
Shipping Condition Dry Ice
Target Entry T3RB, TR beta, THRB, NR1A2
Product Line LanthaScreen
For Use With (Application) Co-Factor Interaction Assay, TR-FRET
Ligand TR Beta
Product Type TR-FRET TR Beta Coactivator Assay Kit
No. of Assays 800 x 20 μL Assays
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For Research Use Only. Not for use in diagnostic procedures.

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