Invitrogen™ Human Lck, His Tag Recombinant Protein

Catalog No.	Quantity
50-274-0565	10 μg
PR4170A	100 μg
PV4081	1 mg

Catalog No. 50-274-0565 Supplier Invitrogen™ Supplier No. P3043

Description

Recombinant Protein

Specific Activity: 240 nmoles of phosphate transferred to poly [Glu, Tyr] 4:1 substrate per minute per mg of total protein at 30°C.

LCK is a member of the Src-family tyrosine kinase, which are essential for signaling through the T-cell receptor (TCR) complex. Upon TCR triggering, LCK phosphorylates the ITAM motives in its zeta subunits establishing binding sites for the SH2 domains of the tyrosine kinase ZAP70, which is also phosphorylated by LCK and activated to generate subsequent signaling platforms by phosphorylation of adaptor LAT. The majority of LCK is localized to the plasma membrane, however, there is also a significant fraction associated with the Golgi apparatus which may contribute to Raf activation under conditions of weak stimulation through the TCR. LCK contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. LCK is also involved in the regulation of apoptosis induced by various stimuli, but not by the death receptors. Diseases associated with LCK include Immunodeficiency 22 and CD45 deficiency. Alternatively splice variants of the LCK gene encoding different isoforms of the protein have been found.

Specifications

• Accession Number: P06239
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50mM tris with 100mM NaCl, 0.05mM EDTA, 0.05% NP-40, 1mM DTT, 50% glycerol and no preservative; pH 7.5
• Gene ID (Entrez): 3932
• Molecular Weight (g/mol): 60.6 kDa
• Name: Human LCK, HIS Tag
• pH Range: 7.5
• Purification Method: Purified
• Quantity: 10 μg
• Storage Requirements: -80°C
• Regulatory Status: RUO
• Gene Alias: EC 2.7.10.2; Hck-3; IMD22; kinase Lck; Lck; LCK proto-oncogene, Src family tyrosine kinase; Lck1; Lcktkr; leukocyte C-terminal Src kinase; LSK; Lskt; Lsk-t; Lymphocyte cell-specific protein-tyrosine kinase; lymphocyte protein tyrosine kinase; lymphocyte-specific protein tyrosine kinase; OTTHUMP00000008640; OTTHUMP00000008740; OTTHUMP00000008741; p56(LSTRA) protein-tyrosine kinase; p56>lck>; p56lck; p56-LCK; pp58lck; Protein YT16; Proto-oncogene Lck; Proto-oncogene tyrosine-protein kinase LCK; RP4-675E8.4; t cell-specific protein-tyrosine kinase; T-lymphocyte specific protein tyrosine kinase p56lck; tyr; tyrosine-protein kinase Lck; YT16
• Common Name: LCK
• Gene Symbol: LCK
• Kinase Family: SRC
• Biological Activity: 240 nM of phosphate transferred to poly [Glu, Tyr] 4:1 substrate / minute / mg of total protein at 30°C. Activity determined (at a final protein concentration of 2 μg/mL)
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-tag
• Content And Storage: -80°C
• Activity: 240 nM of phosphate transferred to poly [Glu, Tyr] 4:1 substrate per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 2 μg/mL
• Expression System: Insect cells
• Protein Family: Kinase and inhibitors
• Form: Liquid
• Protein Subtype: Cytoplasmic tyrosine kinase
• Purity or Quality Grade: 70% as determined by a SDS-PAGE