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Apexbio Technology LLC LDH Cytotoxicity Assay Kit(Synonyms: LDH Cytotoxicity Detection Kit, LDH Release Assay Kit, Lactate Dehydrogenase Cytotoxicity Assay), 1000T.
Supplier: Apexbio Technology LLC K22281000
The LDH Cytotoxicity Assay Kit is a biochemical assay designed to quantitatively assess cell cytotoxicity through measurement of lactate dehydrogenase (LDH) enzyme activity released into the cell culture medium LDH an intracellular enzyme ubiquitously expressed is released extracellularly upon damage or death of the cell membrane This assay relies on LDH-mediated conversion of lactate to pyruvate coupled with NAD reduction to NADH The reaction product NADH interacts with specific substrates within the kit generating a compound measurable spectrophotometrically at 490 nm Accordingly absorbance intensity at this wavelength directly correlates with cellular damage severity Compared to radioactive chromium ( 51Cr) release assays this method provides comparable quantitative data in a safer non-radioactive format applicable for cell death cytotoxicity and drug screening research
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