PAN-Biotech Leibovitz's L-15 Medium, w: L-Glutamine, w/o: NaHCO3 (500 ml). BICARBONATE: Without Sodium Bicarbonate. GLUCOSE: Without glucose. GLUTAMINE: With L-glutamine. PHENOL RED: With Phenol Red. HEPES: Without HEPES. SODIUM PYRUVATE: With sodium pyruvate. STERILE: Yes. STORAGE TEMP: +2-8°C. MANUFACTURER: PAN-Biotech GmbH. COUNTRY OF ORIGIN: Germany. DESCRIPTION: Leibovitz's L-15 Medium was originally developed for use in carbon dioxide (CO2) free systems, and thus does not contain sodium bicarbonate. Instead it is buffered by phosphates and a high concentration of amino acids. Glucose is replaced with galactose and sodium pyruvate, preventing the formation of acid metabolite byproducts. APPLICATION: The L-15 medium supports the growth of established cell lines such as HEP-2, monkey kidney cells and primary explants of embryonic and adult human tissue. With 10% tryptose phosphate broth it is also ideally suited for the cultivation of insect cell lines.