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Sigma Aldrich Fine Chemicals Biosciences Lenti CRISPR Universal Non1EA
Supplier: Sigma Aldrich Fine Chemicals Biosciences CRISPR18V1EA
The Lenti CRISPR Non-Target Control1 Transduction Particles (LV04 vector) include a gRNA sequence that does not target known human mouse and rat genes. The non-target control1 transduction particles are useful as a negative control in experiments using CRISPR lentiviral clones and for cell types that are not amenable to transfection. The Lenti CRISPR Non-Target Contro1 usesl a dual-component system consisting of U6-driven guide RNA (non-target1) and hPGK-driven Puromycin resistance cassette and Blue fluorescence Protein for fluorophorescence. This control requires Cas9 be delivered by a separate vector.Lentiviral-based particles permit efficient infection and integration of the construct into differentiated and non-dividing cells such as neurons and dendritic cells overcoming low transfection and integration difficulties when using these cell lines. Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible
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