Letheen Broth Base (no tween) is used with Tween 80 (Polysorbate 80) for the testing of samples containing quaternary ammonium compounds for antimicrobial activity in a laboratory setting. Letheen Broth Base (no tween) is not intended for use in the diagnosis of disease or other conditions in humans.In 1948, Weber and Black described the value of a highly nutritional solid medium containing neutralizing agents for quaternary ammonium compounds in sanitizers. The addition of Lecithin and Polysorbate 80 to Tryptone Glucose Extract (TGE) Agar resulted in a medium that effectively neutralizes quaternary ammonium compounds while testing germicidal activity. Letheen Agar is a modification of TGE Agar with the addition of Lecithin and Polysorbate 80.Letheen Broth Base was developed as a subculture medium for the neutralization of quaternary ammonium compounds in disinfectant testing.For more information visit https://www.neogen.com/solutions/Microbiology/letheen-broth-base-without-tween