Invitrogen™ Multispecies Leukotriene B4 Competitive ELISA Kit

Catalog No.	Quantity
ENEHLTB4	96 Tests

Catalog No. ENEHLTB4 Supplier Invitrogen™ Supplier No. EHLTB4

Description

ELISA

The Multispecies Leukotriene B4 (LTB4) ELISA quantitates LTB4 in human saliva, urine, porcine plasma and tissue culture medium. Principle of the method This LTB4 competitive ELISA kit is an immunoassay for the quantitative determination of leukotriene B4 (LTB4) in biological fluids. The assay is based on the competition between LTB4 in the standard or sample and alkaline phosphatase conjugated LTB4 (LTB4-AP) for a limited amount of LTB4 monoclonal antibody bound to an anti-Rabbit IgG precoated 96-well plate. As the concentration of LTB4 in the sample increases, the amount of LTB4-AP captured by the coating antibody decreases. Thus, there is an inverse relationship between optical density (OD) and the amount of analyte in the sample. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Leukotriene B4 (LTB4) is a leukotriene involved in inflammation. It is produced from leukocytes in response to inflammatory mediators and is able to induce the adhesion and activation of leukocytes on the endothelium, allowing them to bind and cross into the tissue. In neutrophils, LTB4 is a potent chemoattractant, and is able to induce the formation of reactive oxygen species and the release of lysosomal enzymes.

Specifications

• Assay Range: 11.7-120000 pg/mL
• Assay Sensitivity: <5.63 pg/mL
• Content And Storage: Sufficient For: 96 microplate wells
  • Anti-Rabbit IgG Precoated 96-well Plate, 1 each
  • Reagent Diluent, 30 mL
  • LTB4 Antibody, 6 mL
  • LTB4-AP Conjugate, 6 mL
  • LTB4 Standard, 0.5 mL
  • 10X Wash Buffer, 30 mL
  • Substrate Solution, 20 mL
  • Stop Solution, 6 mL
  • Plate Sealer, 1 each
  • LTB4 Assay Layout Sheet, 1 each
• Description: LTB4 Competitive ELISA Kit
• Conjugate: Alkaline Phosphatase (AP)
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Cytokines & Receptors
• Sample Type: Plasma, Saliva, Supernatant, Urine
• Specificity: No significant cross-reaction with PGE2, PGF2a, 20-OH-LTB4, 20-COOH-LTB4, LTC4, LTD4, LTE4, 5(S)-HETE, 12(S)-HETE, 15(S)-HETE; less than 1% cross-reaction with 12-epi-LTB4; approx. 5% cross-reaction with 6-trans-LTB4 and 6-trans-12-epi-LTB4
• Sufficient For: 96 microplate wells
• Target Species: Human, Pig
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: BLT; BLT1; BLTR; Chemoattractant receptor-like 1; chemokine receptor-like 1; CMKRL1; G protein-coupled receptor 16; GPR16; G-protein coupled receptor 16; leukotriene B4 receptor; leukotriene B4 receptor 1; LTB4R; LTB4-R 1; LTB4R1; LTB4-R1; LTBR1; P2RY7; P2Y purinoceptor 7; P2Y7; purinergic receptor P2Y, G-protein coupled, 7BLT; BLT1; BLTR; Chemoattractant receptor-like 1; chemokine receptor-like 1; CMKRL1; G protein-coupled receptor 16; GPR16; G-protein coupled receptor 16; leukotriene B4 receptor; leukotriene B4 receptor 1; LTB4R; LTB4-R 1; LTB4R1; LTB4-R1; LTBR1; P2RY7; P2Y purinoceptor 7; P2Y7; purinergic receptor P2Y, G-protein coupled, 7;
• Interassay CV: 12.4%
• Intraassay CV: 6.5%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent, Detection Antibody, AP Conjugate, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent, Detection Antibody, AP Conjugate, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: AP
• Measurement: Absorbance at 405 nm minus absorbance (at 570 nm)
• Precision: Dependent upon sample type and level
• Protein Aliases: LTB4; C20H32O4
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Saliva 25 μL, Supernatant 100 μL, Urine 100 μL
• Storage Requirements: -20°C
• Target: Leukotriene B4
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov