Invitrogen™ Levonorgestrel (LNG) Competitive ELISA Kit

Catalog No.	Quantity
EIALNGCX10	10 x 96 Tests
EIALNGC	96 Tests

Catalog No. EIALNGCX10 Supplier Invitrogen™ Supplier No. EIALNGCX10

Description

ELISA

The Levonorgestrel (LNG) ELISA quantitates LNG in serum,plasma,urine,dried fecal extracts,saliva,milk,or cell culture medium.

Principle of the method

The Levonorgestrel (LNG) Competitive ELISA research-use-only kit is designed to quantitatively measure levonorgestrel present in serum,plasma,saliva,urine,dried fecal samples,milk,and tissue culture media samples. This kit measures total LNG in extracted serum,plasma,milk and fecal samples. A levonorgestrel stock solution is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. A levonorgestrel-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a rabbit polyclonal antibody to levonorgestrel to each well. After a 1-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound levonorgestrel-peroxidase conjugate. After a short incubation,the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.

Levonorgestrel (4-Estren-17alpha-Ethynyl-18-homo-17beta-ol-3-one (LNG) is a synthetic steroid analogue.

Specifications

• Assay Range: 6.25-200 pg/mL
• Assay Sensitivity: 2.2 pg/mL
• Description: Levonorgestrel (LNG) Competitive ELISA Kit
• Assay: Multiplex Assay on the Luminex Platform
• Conjugate: HRP
• Detection Method: Fluorescence
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Fecal Extract, Milk, Plasma, Saliva, Serum, Supernatant, Urine, Water
• Sufficient For: 10 x 96 Assays
• Target Species: Chemical
• For Use With (Application): ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti
• For Use With (Equipment): Absorbance Microplate Reader
• Interassay CV: 9.1%
• Intraassay CV: 9%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, HRP-Conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, HRP-Conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Protein Aliases: C21H28O2
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Research Discipline: Reproductive Biology
• Sample Volume: Fecal Extract 100 μL, Milk 100 μL, Plasma 100 μL, Saliva 50 μL, Serum 100 μL, Supernatant 100 μL, Urine 25 μL, Water 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: -20°C
• Target: Levonorgestrel
• Test Time: 1 hr 20 min
• Total Assay Time: 1 hr 30 min