Thermo Scientific™ LightShift™ Chemiluminescent RNA EMSA Kit

Description

Study RNA-protein interactions with this non-radioactive EMSA kit for gel shift assays.

Thermo Scientific™ LightShift Chemiluminescent RNA EMSA Kit provides a non-radioactive solution for studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA).

The RNA EMSA Kit uses biotinylated RNA probes and a chemiluminescent substrate system to achieve fast and safe detection of RNA-protein complexes with sensitivity equivalent to traditional ³²P-isotopic methods. The complete kit comes with all reagents required to set up and optimize protein-RNA binding conditions, a positive control for protein-RNA interactions and reagents for chemiluminescent detection of the nucleic acid interaction.

Highlights:

• Sensitive – chemiluminescent detection comparable to radioactive detection

• Time-saving – develop X-ray films after 1- to 5-minute exposures, versus 16 hour exposures needed with radioactive systems

• Flexible – compatible with RNA probes biotinylated by different methods

• Easy to use – complete kit includes optimized reagents for binding reactions and RNA probe detection

• Non-radioactive – eliminate waste concerns from radioactive RNA probes

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Specifications

• Assay: EMSA Assay
• Content And Storage: Sufficient For: 100 binding reactions and detection on 1000 cm² of membrane (10 mini-gel blots)• 125 nM Biotinylated IRE RNA Control 35 μL (store at -20°C)
  • 10 μM Unlabeled IRE RNA Control, 25 μL (store at -20°C)
  • 2 mg/mL Cytosolic Liver Extract, 50 μL (store at -20°C)
  • 10 mg/mL tRNA, 100 μL (store at -20°C)
  • 10X REMSA Binding Buffer, 1 mL (store at -20°C)
  • 50% Glycerol, 500 μL (store at -20°C)
  • 2M KCl, 500 μL (store at -20°C)
  • 1M MgCl₂, 500 μL (store at -20°C)
  • DTT, lyophilized (store at -20°C)
  • Nuclease-Free Water, 1.5 mL (store at -20°C)
  • 5X REMSA Loading Buffer, 1 mL (store at -20°C)
  • Stabilized Streptavidin-HRP, 1.5 mL (store at 4°C)
  • Luminol/Enhancer Solution, 80 mL (store at 4°C)
  • Stable Peroxide Solution, 80 mL (store at 4°C)
  • Nucleic Acid Detection Blocking Buffer, 500 mL (store at 4°C)
  • 4X Wash Buffer, 500 mL (store at 4°C)
  • Substrate Equilibration Buffer, 500 mL (store at 4°C)
• Detection Method: Chemiluminescence
• Format: Kit
• Sample Type: Protein
• For Use With (Equipment): myECL™ Imager, X-Ray Film
• No. of Reactions: 100 reactions
• Product Line: LightShift™
• Product Type: LightShift Chemiluminescent RNA EMSA Kit
• Quantity: 100 reactions
• Target Specificity: Not Target-Specific
• Technique: Enhanced Chemiluminescence, Membrane Blot, Gel Shift

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.