Thermo Scientific LightShift Chemiluminescent RNA EMSA Kit provides a non-radioactive solution for studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA).
The RNA EMSA Kit uses biotinylated RNA probes and a chemiluminescent substrate system to achieve fast and safe detection of RNA-protein complexes with sensitivity equivalent to traditional 32P-isotopic methods. The complete kit comes with all reagents required to set up and optimize protein-RNA binding conditions, a positive control for protein-RNA interactions and reagents for chemiluminescent detection of the nucleic acid interaction.
Sensitive chemiluminescent detection comparable to radioactive detection
Time-saving develop X-ray films after 1- to 5-minute exposures, versus 16 hour exposures needed with radioactive systems
Flexible compatible with RNA probes biotinylated by different methods
Easy to use complete kit includes optimized reagents for binding reactions and RNA probe detection
Non-radioactive eliminate waste concerns from radioactive RNA probes
100 binding reactions using typical conditions
Studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA)
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