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Thermo Scientific™ LightShift™ Chemiluminescent RNA EMSA Kit

Study RNA-protein interactions with this non-radioactive EMSA kit for gel shift assays.

Manufacturer:  Thermo Scientific™ 20159

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Catalog No. 20-159-MI


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Specifications

Description

Thermo Scientific™ LightShift Chemiluminescent RNA EMSA Kit provides a non-radioactive solution for studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA).

The RNA EMSA Kit uses biotinylated RNA probes and a chemiluminescent substrate system to achieve fast and safe detection of RNA-protein complexes with sensitivity equivalent to traditional 32P-isotopic methods. The complete kit comes with all reagents required to set up and optimize protein-RNA binding conditions, a positive control for protein-RNA interactions and reagents for chemiluminescent detection of the nucleic acid interaction.

Highlights:

  • Sensitive – chemiluminescent detection comparable to radioactive detection

  • Time-saving – develop X-ray films after 1- to 5-minute exposures, versus 16 hour exposures needed with radioactive systems

  • Flexible – compatible with RNA probes biotinylated by different methods

  • Easy to use – complete kit includes optimized reagents for binding reactions and RNA probe detection

  • Non-radioactive – eliminate waste concerns from radioactive RNA probes

Description & Specifications

Specifications

Studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA)
100 binding reactions using typical conditions
100µL
10mg/mL solution
tRNA for LightShift RNA EMSA Kit
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