Thermo Scientific™ LightShift™ Chemiluminescent RNA EMSA Kit

Study RNA-protein interactions with this non-radioactive EMSA kit for gel shift assays.

Manufacturer: Thermo Scientific™ 20159

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Catalog No. 20-159-MI

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    Specifications

    For Use With (Application) Studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA)
    Formulation 10mg/mL solution
    No. of Reactions 100 binding reactions using typical conditions
    Product Type tRNA for LightShift RNA EMSA Kit
    Quantity 100µL

    Thermo Scientific™ LightShift Chemiluminescent RNA EMSA Kit provides a non-radioactive solution for studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA).

    The RNA EMSA Kit uses biotinylated RNA probes and a chemiluminescent substrate system to achieve fast and safe detection of RNA-protein complexes with sensitivity equivalent to traditional 32P-isotopic methods. The complete kit comes with all reagents required to set up and optimize protein-RNA binding conditions, a positive control for protein-RNA interactions and reagents for chemiluminescent detection of the nucleic acid interaction.

    Highlights:

    • Sensitive – chemiluminescent detection comparable to radioactive detection

    • Time-saving – develop X-ray films after 1- to 5-minute exposures, versus 16 hour exposures needed with radioactive systems

    • Flexible – compatible with RNA probes biotinylated by different methods

    • Easy to use – complete kit includes optimized reagents for binding reactions and RNA probe detection

    • Non-radioactive – eliminate waste concerns from radioactive RNA probes

  • Description & Specifications

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