Thermo Scientific™ LightShift™ Chemiluminescent RNA EMSA Kit

Study RNA-protein interactions with this non-radioactive EMSA kit for gel shift assays.

Manufacturer: Thermo Scientific™ 20159

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Catalog No. 20-159-MI

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    For Use With (Application) Studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA)
    Formulation 10mg/mL solution
    No. of Reactions 100 binding reactions using typical conditions
    Product Type tRNA for LightShift RNA EMSA Kit
    Quantity 100µL,100µL
    Product Name tRNA for LightShift RNA EMSA Kit
    Sufficient For 100 binding reactions using typical conditions

    Thermo Scientific™ LightShift Chemiluminescent RNA EMSA Kit provides a non-radioactive solution for studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA).

    The RNA EMSA Kit uses biotinylated RNA probes and a chemiluminescent substrate system to achieve fast and safe detection of RNA-protein complexes with sensitivity equivalent to traditional 32P-isotopic methods. The complete kit comes with all reagents required to set up and optimize protein-RNA binding conditions, a positive control for protein-RNA interactions and reagents for chemiluminescent detection of the nucleic acid interaction.


    • Sensitive – chemiluminescent detection comparable to radioactive detection

    • Time-saving – develop X-ray films after 1- to 5-minute exposures, versus 16 hour exposures needed with radioactive systems

    • Flexible – compatible with RNA probes biotinylated by different methods

    • Easy to use – complete kit includes optimized reagents for binding reactions and RNA probe detection

    • Non-radioactive – eliminate waste concerns from radioactive RNA probes

  • Description & Specifications

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