Invitrogen™ Human LIMK2, GST Tag Recombinant Protein

Catalog No.	Quantity
PV4349	1 mg
PR7519B	100 μg
PV3860	10 μg

Catalog No. PV4349 Supplier Invitrogen™ Supplier No. PV4349

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Protein Form: Full Length.

LIMK2 is a serine/threonine kinase involved in regulation of actin cytoskeletal changes via phosphorylation of cofilin. LIMK2 has also been implicated in brain development. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by LIMK2 is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for LIMK2.

Specifications

• Accession Number: P53671
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50mM tris with 0.5mM EDTA, 50% glycerol, 2mM DTT, 0.02% Triton X-100, 150mM NaCl and no preservative; pH 7.5
• Gene ID (Entrez): 3985
• Molecular Weight (g/mol): 99.7 kDa
• Name: Human LIMK2, GST Tag
• pH Range: 7.5
• Purification Method: Purified
• Quantity: 1 mg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: EC 2.7.11.1; kinase LIMK2; LIM domain kinase 2; LIM kinase 2; LIM motif-containing protein kinase 2; LIMK; LIMK 2; LIMK1; LIMK-1; Limk2; LIMK-2; Limk2b; LINhp-2; Link2; OTTHUMP00000199483
• Common Name: LIMK2
• Gene Symbol: LIMK2
• Kinase Family: LISK Kinase Family
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: GST-tag
• Sequence: Full length
• Content And Storage: -80°C, Avoid Freeze/Thaw Cycles
• Kinase Group: Ser/Thr Kinases: TKL Group
• Shipping Condition: Dry Ice
• Expression System: Baculovirus
• Protein Family: Kinases & Inhibitors
• Protein Form: Full Length
• Form: Liquid
• Research Category: Drug Development