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Cell Biolabs Inc Lipid Doplet Isolation Kit, 50 preps
Supplier: Cell Biolabs Inc MET5011

Lipid droplets are organelles that are rich in lipids, contain a lipid rich core, and are surrounded by a phospholipid monolayer and associated proteins. They are found in adipose tissue of animals and regulate the hydrolysis and storage of neutral lipids; they also serve as storage for cholesterol and acyl-glycerols used to form cell membranes. More recently they have been associated with inflammatory responses, obesity, atherosclerosis, and cancer. Cell Biolabs’ Lipid Droplet Isolation Kit isolates lipid droplets by simple gradient centrifugation, eliminating the need for ultracentrifugation. A lipid droplet source such as tissue or cultured cells is homogenized. A gradient is then created with the homogenate, and the material is centrifuged. The lipid droplets float to the top of the gradient and are recovered by carefully pipetting from the top of the gradient. Each kit provides sufficient reagents to isolate up to 50 preps based on a 50-100 mg tissue or cell sample size.
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