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Biotium Live-or-Dye NucFix™ Red Staining Kit (200 assays)
Supplier: Biotium 32010
Live-or-Dye NucFix™ Red is a chemically-reactive, cell membrane impermeable dye that specifically stains the nuclei of dead cells. The dye is able to enter into dead cells that have compromised membrane integrity and covalently label the cell nucleus, allowing for clear differentiation of live and dead cells by either microscopy or flow cytometry. Unlike other commonly used nuclear stains such as Propidium Iodide or DRAQ7™, NucFix™ labeling is extremely stable, allowing the cells to be fixed and permeabilized without loss of fluorescence or dye transfer between cells. The Live-or-Dye NucFix™ Red staining protocol has been optimized to maximize live/dead discrimination with minimal live cell staining, in order to prevent interference with immunostaining. The dye has an excitation/emission of 520/593 nm and is suitable for staining mammalian cells, yeast, or gram-negative bacteria. This kit contains sufficient reagent for 200 assays.
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