Invitrogen™ Mouse LIX/CXCL5 ELISA Kit

Catalog No.	Quantity
EMCXCL5	96 Tests
EMCXCL5X5	5 x 96 Tests

Catalog No. EMCXCL5 Supplier Invitrogen™ Supplier No. EMCXCL5

Description

ELISA

The Mouse LIX (CXCL5) ELISA quantitates Ms LIX in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms LIX. Principle of the method The Mouse LIX solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The protein encoded by this gene is an inflammatory chemokine that belongs to the CXC chemokine family. This chemokine is produced concomitantly with interleukin-8 (IL8) in response to stimulation with either interleukin-1 (IL1) or tumor necrosis factor-alpha (TNFA). This chemokine is a potent chemotaxin involved in neutrophil activation.

Specifications

• Accession Number: P50228
• Assay Range: 35-2000 pg/mL
• Assay Sensitivity: 35 pg/mL
• Calibration Range: 35 pg/mL to 2000 pg/mL
• Content And Storage: Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.
• Assay: Solid-phase sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Chemokines & Receptors
• Sample Type: Plasma, Serum, Supernatant
• Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), L-Selectin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
• Sufficient For: 96 Assays
• Target Species: Mouse
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: AMCF-II; C Cmotif chemokine; C X C motif chemokine; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 5; chemokine (C-X-C motif) ligand 6; chemokine (C-X-C motif) ligand 6 (granulocyte chemotactic protein 2); CXC; CXC chemokine LIX; CXC motif chemokine; C-X-C motif chemokine 5; C-X-C motif chemokine ligand 5; CXCL; CXCL5; Cxcl6; Cytokine LIX; ENA78; ENA-78; ENA-78(1-78); ENA-78(8-78); ENA-78(9-78); Epithelial-derived neutrophil-activating protein 78; GCP-2; GCP-2(1-78); GCP-2(9-78); granulocyte chemotactic protein-2; LIX; Neutrophil-activating peptide ENA-78; neutrophil-activating protein 78; Scyb5; Scyb6; small inducible cytokine B subfamily, member 5; small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78); small inducible cytokine subfamily B, member 15; small-inducible cytokine B5AMCF-II; C Cmotif chemokine; C X C motif chemokine; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 5; chemokine (C-X-C motif) ligand 6; chemokine (C-X-C motif) ligand 6 (granulocyte chemotactic protein 2); CXC; CXC chemokine LIX; CXC motif chemokine; C-X-C motif chemokine 5; C-X-C motif chemokine ligand 5; CXCL; CXCL5; Cxcl6; Cytokine LIX; ENA78; ENA-78; ENA-78(1-78); ENA-78(8-78); ENA-78(9-78); Epithelial-derived neutrophil-activating protein 78; GCP-2; GCP-2(1-78); GCP-2(9-78); granulocyte chemotactic protein-2; LIX; Neutrophil-activating peptide ENA-78; neutrophil-activating protein 78; Scyb5; Scyb6; small inducible cytokine B subfamily, member 5; small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78); small inducible cytokine subfamily B, member 15; small-inducible cytokine B5; AMCF-II; Cxcl6; ENA-78; GCP-2; LIX; Scyb5; Scyb6
• Gene ID (Entrez): 20311
• Gene Symbol: CXCL5
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Protein Aliases: Cytokine LIX, Small-inducible cytokine B5
• Quantity: 96 Tests
• Regulatory Status: RUO
• Research Discipline: Inflammation
• Sample Volume: Plasma 20 μL, Serum 20 μL, Supernatant 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Synonym: AMCF-II, ENA-78, GCP-2, LIX, Scyb5, Scyb6
• Target: CXCL5
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min