Invitrogen™ LIX/CXCL5 Mouse ELISA Kit

Sandwich ELISA Kit

Manufacturer:  Invitrogen™ EMCXCL5

Catalog No. EMCXCL5

Description

The Mouse LIX (CXCL5) ELISA quantitates Ms LIX in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms LIX. Principle of the method The Mouse LIX solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CXCL5 is a member of the CXC subfamily of chemokines. The cDNA encodes a 114 amino acid precursor protein which when cleaved generates a 36 amino acid signal peptide and a 78 amino acid mature protein which contains a 12 amino acids sequence found before the CXC motif. CXCL5 is produced by various human epithelial cells and it binds to the same receptors as IL8 and NAP2.

Specifications

• Sufficient For: 96 assays
• Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), L-Selectin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
• Conjugate: Biotin
• Assay: Solid-phase sandwich ELISA
• Accession Number: P50228
• Calibration Range: 35pg/mL to 2000pg/mL
• Gene ID (Entrez): 20311
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Total Assay Time: 4 hr. 45 min.
• Research Discipline: Inflammation
• Interassay CV: <12%
• Quantity: 96 Tests
• Sample Type: Plasma, Serum, Supernatant
• Gene Symbol: AMCF-II,Cxcl6,ENA-78,GCP-2,LIX,Scyb5,Scyb6

• Product Type: ELISA Kit
• For Use With (Equipment): Colorimetric Microplate Reader
• Protein Family: Chemokine & Receptors
• Label or Dye: HRP
• Assay Range: 31.25-2000 pg/mL
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Sample Volume: Plasma, 20 μL; Serum, 20 μL; Supernatant, 50 μL
• Test Time: 1 hr. 20 min.
• Target: Mouse
• Synonym: AMCF-II, ENA-78, GCP-2, LIX, Scyb5, Scyb6
• Intraassay CV: <10%
• Target Species: Mouse
• Assay Sensitivity: 35 pg/mL