New England Biolabs, Inc. LongAmp Hot Start Taq 2X Master Mix – 100 reactions (50 µl vol)

Supplier:  New England Biolabs, Inc. M0533S

LongAmp Hot Start Taq 2X Master Mix contains a unique blend of aptamer-based Hot Start Taq and Deep Vent DNA Polymerases. The aptamer-based inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45C, but releases the enzyme during normal PCR cycling conditions. This permits assembly of PCR reactions at room temperature. The LongAmp Hot Start Taq 2X Master Mix does not require a separate high temperature incubation step to activate the enzyme. The 3' tp 5' exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Hot Start Taq. LongAmp Hot Start Taq DNA Polymerase offers two-fold higher fidelity than Hot Start Taq DNA Polymerase alone. The convenient master mix formulation is supplied at a 2X concentration and contains dNTPs and Mg++, requiring only the addition of primers and DNA template for robust amplification. A wide range of PCR products can be generated; up to 30 kb from lambda or human genomic DNA.

Catalog No. 50-591-073