Invitrogen™ Low Input RiboMinus™ Eukaryote System v2

Description

The Low Input RiboMinus™ Eukaryote System v2 improves upon the previous system (RiboMinus™ Eukaryote Kit for RNA-Seq) by removing significantly more rRNA (both cytoplasmic and mitochondrial) from the total RNA.

Features of the system include:
• Magnetic bead-based purification for rapid, seamless purification
• 1-hour workflow
• Low input range of 100 ng–1 μg

The Low Input RiboMinus™ Eukaryote System v2 improves upon the previous system (RiboMinus™ Eukaryote Kit for RNA-Seq) by removing significantly more rRNA (both cytoplasmic and mitochondrial) from the total RNA. When used with our library construction kits for sequencing, the Low Input RiboMinus™ Eukaryote System v2 provides maximum mapping to reference database with minimum bias, maintaining relative gene expression levels of even smaller RNA species. The resulting total RNA after depletion contains minimum contaminating rRNA with reduced variability.

The Low Input RiboMinus Eukaryote System v2 is designed for the removal of human, mouse, and rat rRNA. A magnetic bead-based purification module is included for purification of the whole transcriptome without altering the expression levels of small or non-coding RNA. For column-based purification, please see the RiboMinus™ Eukaryote Kit v2. The Low Input RiboMinus Eukaryote System v2 has an RNA input range of 100 ng–1 μg. For high inputs (up to 5 ug), the RiboMinus™ Eukaryote System v2 is recommended.

The Low Input RiboMinus™ Eukaryote System v2 contains sufficient reagents to perform 12 ribosomal RNA depletions.

Specifications

• Content And Storage: • RiboMinus™ Eukaryote Probe Mix, 2-20°C
  • RiboMinus™ Magnetic Beads, 4°C
  • 2 x Hybridization Buffer, room temperature
  • Binding Solution Concentrate: room temperature
  • Wash Solution Concentrate, room temperature
  • Nucleic Acid Binding Beads: 4°C
• Species: Human, Mouse, Rat
• Final Product Type: Transcriptome RNA
• For Use With (Equipment): Ion PGM™ System, Ion Proton™ System, Illumina™ Genome Analyzer II (GA II)
• No. of Reactions: 12 reactions
• Product Line: RiboMinus™
• Quantity: 12 reactions
• Sequencing Type: Transcriptome Sequencing
• Starting Material Amount: 100 ng - 1 μg total RNA
• Workflow Step: Sample Enrichment

For Research Use Only. Not for use in diagnostic procedures.