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Enzo Life Sciences LSD1 fluorometric drug discovery kit (1x96wells)
Supplier: Enzo Life Sciences BMLAK5440001
The LSD1 Fluorimetric Drug Discovery Kit provides human recombinant LSD1 and all reagents for measuring its activity in a sensitive, real-time fluorescent assay. LSD1 catalyzed demethylation of the Histone H3 Dimethyl Lysine-4 Peptide (H3K4Me2 Peptide; BML-P256) produces hydrogen peroxide. A fluorescent signal is generated via the horseradish peroxidase (HRP) catalyzed reaction of the hydrogen peroxide with the CELLESTIAL Red Peroxidase Substrate (BML-KI565). Although fluorescence detection will be more sensitive (Excitation in range of 530-570 nm; Emission ca. 590 nm), the CELLESTIAL Red peroxidation product may also be detected by following absorbance (see "Detection by Absorbance at 563 nm"). Alternative name: Lysine-specific Histone Demethylase 1, KDM1, AOF2. Applications: Fluorescent detection, HTS Cell-based assays, Activity assay.
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