Invitrogen™ Human Luteinizing Hormone ELISA Kit

Catalog No.	Quantity
EHLH	96 Tests
EHLHX5	5 x 96 Tests

Catalog No. EHLH Supplier Invitrogen™ Supplier No. EHLH

Description

ELISA

The Human Luteinizing Hormone (Hu LH) ELISA quantitates Hu LH in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu LH. Principle of the method The Human LH solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

LH is a member of the glycoprotein hormone family, Glycoprotein hormones are heterodimers consisting of a common alpha subunit and an unique beta subunit which confers biological specificity. LH is expressed in the pituitary gland and promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids. The genes for the beta chains of chorionic gonadotropin and for luteinizing hormone are contiguous on chromosome 19q13.3. Mutations in this gene are associated with hypogonadism which is characterized by infertility and pseudohermaphroditism.

Specifications

• Accession Number: P01215, P01229
• Assay Range: 140-10000 pg/mL
• Assay Sensitivity: 140 pg/mL
• Calibration Range: 140 pg/mL to 10,000 pg/mL
• Content And Storage: Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.
• Assay: Solid-phase sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Other Proteins
• Sample Type: Plasma, Serum, Supernatant
• Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF-7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF,SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
• Sufficient For: 96 assays
• Target Species: Human
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: aGSU; alphaGSU; alpha-GSU; alphaSU; Anterior pituitary glycoprotein hormones common subunit alpha; CGA; Cga1; CG-alpha; CGB4; Choriogonadotropin alpha chain; chorionic gonadotrophin subunit alpha; chorionic gonadotropin, alpha polypeptide; follicle-stimulating hormone alpha chain; follicle-stimulating hormone alpha subunit; follitropin; follitropin alpha chain; FSHA; FSH-alpha; FSH-B; FSH-beta; glycoprotein hormones alpha chain; glycoprotein hormones, alpha polypeptide; glycoprotein hormones, alpha subunit; GPHa; GPHA1; GPHalpha; HCG; HH23; hLHB; interstitial cell stimulating hormone, beta chain; interstitial cell-stimulating hormone; leutenising hormone beta; leutropin; LH; LH alpha 1-1; LH alpha 1-2; LH alpha 1-3; LH alpha 3; LH beta-1; LH beta-2; LH beta-3; LHA; LHB; LH-B; LHbeta; LSH-alpha; LSH-B; LSH-beta; Luteinizing hormone (lutropin) subunit beta; luteinizing hormone alpha chain; luteinizing hormone beta; luteinizing hormone beta polypeptide; luteinizing hormone beta subunit; luteinizing hormone subunit beta; Lutropin alpha chain; lutropin beta chain; Lutropin subunit beta; pituitary hormone alpha subunit; precursor polypeptide; precursor polypeptide (AA -24 to 96); thyroid-stimulating hormone alpha chain; Thyrotropin alpha chain; TSHA; TSH-alphaaGSU; alphaGSU; alpha-GSU; alphaSU; Anterior pituitary glycoprotein hormones common subunit alpha; CGA; Cga1; CG-alpha; CGB4; Choriogonadotropin alpha chain; chorionic gonadotrophin subunit alpha; chorionic gonadotropin, alpha polypeptide; follicle-stimulating hormone alpha chain; follicle-stimulating hormone alpha subunit; follitropin; follitropin alpha chain; FSHA; FSH-alpha; FSH-B; FSH-beta; glycoprotein hormones alpha chain; glycoprotein hormones, alpha polypeptide; glycoprotein hormones, alpha subunit; GPHa; GPHA1; GPHalpha; HCG; HH23; hLHB; interstitial cell stimulating hormone, beta chain; interstitial cell-stimulating hormone; leutenising hormone beta; leutropin; LH; LH alpha 1-1; LH alpha 1-2; LH alpha 1-3; LH alpha 3; LH beta-1; LH beta-2; LH beta-3; LHA; LHB; LH-B; LHbeta; LSH-alpha; LSH-B; LSH-beta; Luteinizing hormone (lutropin) subunit beta; luteinizing hormone alpha chain; luteinizing hormone beta; luteinizing hormone beta polypeptide; luteinizing hormone beta subunit; luteinizing hormone subunit beta; Lutropin alpha chain; lutropin beta chain; Lutropin subunit beta; pituitary hormone alpha subunit; precursor polypeptide; precursor polypeptide (AA -24 to 96); thyroid-stimulating hormone alpha chain; Thyrotropin alpha chain; TSHA; TSH-alpha; CGB4; HH23; LSH-B; LSH-beta
• Gene ID (Entrez): 1081, 3972
• Gene Symbol: Cga, LHB
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Protein Aliases: Lutropin beta chain, Luteinizing hormone subunit beta, LH-B, LSH-B, LSH-beta
• Quantity: 96 Tests
• Regulatory Status: RUO
• Research Discipline: Signal Transduction
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Synonym: RP11-156F23.1, CG-ALPHA, FSHA, GPHA1, GPHa, HCG, LHA, TSHA
• Target: Luteinizing Hormone
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min