Invitrogen™ LXA4 Competitive ELISA Kit

Catalog No.	Quantity
EEL011	96 Tests

Catalog No. EEL011 Supplier Invitrogen™ Supplier No. EEL011

Description

ELISA

The multispecies Lipoxin A4 (LXA4) ELISA quantitates LXA4 in serum, plasma, and other biological fluids Principle of the method: The Competitive multispecies ELISA research-use-only kit is designed to quantitatively measure Lipoxin A4 (LXA4) independent of species. A LXA4 standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to LXA4is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of LXA4 in the samples is then determined by comparing the OD of the samples to the standard curve. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Lipoxin A4 (LXA4) is a potent lipid mediator that plays a crucial role in resolving inflammation and promoting tissue repair. It is a member of the lipoxin family of eicosanoids, which are derived from arachidonic acid metabolism. LXA4 is primarily produced by leukocytes, including neutrophils, monocytes, and macrophages, in response to inflammatory stimuli. LXA4 exerts its anti-inflammatory and pro-resolving effects by binding to specific receptors on target cells, including ALX/FPR2 and GPR32. These receptors are expressed on various immune cells, endothelial cells, and epithelial cells, and their activation leads to the inhibition of pro-inflammatory signaling pathways and the induction of tissue repair processes. The structure of LXA4 is characterized by a 5S, 6R, 15S-trihydroxy-7E, 9E, 11Z, 13E-eicosatetraenoic acid backbone, with a hydroxyl group at position 17 and a carboxylic acid group at position 16. LXA4 is a stereoisomer of another lipoxin, LXB4, which has an opposite configuration at position 5 and a reduced hydroxyl group at position 15.

Specifications

• Assay Range: 0.78-50 ng/mL
• Assay Sensitivity: 0.47 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Specialized pro-resolving mediators
• Sample Type: Plasma, Serum
• Target Species: All Species
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: Lipoxin A4Lipoxin A4;
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: LXA4
• Test Time: 1 hr 20 min
• Total Assay Time: 2 hr 30 min