Invitrogen™ Lycopersicon Esculentum (Tomato) Lectin (LEL, TL), DyLight 649

Description

Used as marker of blood vessels and microglial cells in rodents and in neuroscience research

• Lycopersicon esculentum (Tomato) Lectin (LEL, TL), DyLight 649, is a bright deep-red/far-red LEL-fluorophore conjugate. The tomato lectin LEL is widely used as marker of blood vessels and microglial cells in rodents and in neuroscience research. It is a valuable tool in studies of rodent tumor angiogenesis and the tracing of neovascular development in xenograft models and brain research.
• LEL-DyLight 649 can be applied directly to tissue sections, or it can be introduced through intravascular perfusion for fluorescence detection.
• Very bright DyLight 649 conjugate detected with traditional Cy5/deep-red filter set, Ex/Em: 655/670 nm
• Applications: immunofluorescence, glycobiology
• Sugar specificity: [GlcNAc] 1,3-N-acetylglucosamine
• Inhibiting/eluting sugar: chitin hydrolysate
• Blood group specificity: non-specific
• Unconjugated lectin molecular weight: 71 KDa.
• Immunofluorescence working concentration: 5–20 μg/mL
• LEL is a very stable single-subunit glycoprotein containing ∼50 percent arabinose and galactose and may form multimeric aggregates in solution. It is provided at a 1 mg/mL concentration in 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM Ca². If a precipitate forms upon long-term storage, warm to 37°C.

Specifications

• Quantity: 1 mg
• Source: Tomato
• Conjugate: DyLight 649
• Content And Storage: When stored at or below 2–8°C in the dark, the product is stable for 6 months after reciept.
  
  
• Label Type: DyLight Fluors
• Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice
• Form: Solution
• Protein Subtype: Lectins
• Product Line: DyLight™

For Research Use Only. Not for use in diagnostic procedures.