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Invitrogen™ Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)
Description
Invitrogen Lyo-ready recombinase polymerase amplification (RPA) reagents are designed for fast, sensitive, and specific DNA amplification through isothermal RPA reactions. Lyo-ready RPA reagents are available in stand-alone and kit formats. Reverse transcriptase can be added to the RPA reaction for amplification of RNA targets. All reagents are glycerol-free, which enables assay lyophilization.
Features of Lyo-ready RPA products
- Enhanced sensitivity down to 1 copy using optimized (RT-)RPA reaction buffer
- Robust and specific RNA/DNA amplification
- Amplification from inhibitor-containing samples
- Fast target amplification in as little as 20 minutes
- Ability to easily optimize RPA or RT-RPA reactions
Kit components
The Lyo-ready RPA Kit includes all required components for an RPA reaction:
- Lyo-ready T4 UvsX Protein—produced from E. coli cells bearing cloned uvsX gene derived from bacteriophage T4
- Lyo-ready T4 UvsY Protein—a recombination mediator protein that stimulates homologous recombination performed by the UvsX recombinase
- Lyo-ready Bst DNA Polymerase (Cat. No. A56655)—a proprietary mutant of Bst DNA Polymerase, Large Fragment, with fast reaction speed, increased sensitivity, and tolerance to inhibitors
- Lyo-ready T4 Gene 32 Protein—a single-stranded DNA binding protein produced from E. coli cells carrying cloned bacteriophage T4 gene 32
- Optimized reaction buffer, magnesium chloride, and dNTPs
Specifications
Specifications
| Product Type | Lyo-ready T4 Gene 32 Protein |
| Form | Liquid |
| For Use With (Application) | RPA, RT-RPA, cDNA Synthesis, PCR |
| Quantity | 1600 μg |
| Research Category | Nucleic Acid Amplification |
| Content And Storage | Lyo-ready T4 Gene 32 Protein, 80 μL, Store at -25°C to -15°C. |
| Shipping Condition | Dry Ice |
| Concentration | 20 mg/mL |
For Research Use Only. Not for use in diagnostic procedures.
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