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ILEX LIFE SCIENCES LLC Immuchrom Lysozyme ELISA Kit for Human Stool Samples, 96 wells

Supplier: ILEX LIFE SCIENCES LLC IC6900
The ImmuChrom Lysozyme ELISA Kit is an immunoassay intended for the quantitative determination of lysozyme in human stool samples. Calibration Range: 0.55 ng/ml - 5 ng/ml. Detection Limit: 150 ng/g. Principle: The test determines human lysozyme according to the sandwich-principle. Lysozyme in sample, standard and controls binds to antibodies, which are coated to the microtiter plate. After a washing step a peroxidase labeled detection antibody is added. A second washing step is followed by the addition of the substrate which is converted to a colored product by the peroxidase. The reaction is terminated by the addition of an acidic stop solution. The optical densities are read at 450 nm (against the reference wavelength 620 nm) in a microtiter plate reader. The lysozyme concentration can be calculated from the standard curve. Synonyms: Muramidase, N-acetylmuramide glycanhydrolase, EC 3.2.1.17, LYZL1 gene product, faecal lysozyme quantification, fecal lysozyme enzyme.
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