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MP Biomedicals MagBeads Plasmid Miniprep

Catalog No. MP116587050 Shop All MP Biomedicals, Inc Products
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Description

Ready-to-use prefilled format for MPure™ systems, ideal for both high and low copy plasmids.

  • The MagBeads Plasmid Miniprep Kit offers a scalable and automatable purification method using magnetic bead technology, suitable for extracting both high- and low-copy-number plasmids. This kit efficiently yields plasmid DNA (2 - 20 μg) from 0.5 - 5 mL of overnight E. coli cultures, utilizing paramagnetic beads for rapid processing. Its magnetic technology enhances user-friendliness, making it ideal for both small sample sizes and high-throughput automated nucleic acid purification.
  • The kit includes components for cell lysis and neutralization. Bacterial cells are first resuspended in Resuspension Buffer. Following resuspension, the cells are lysed with Alkaline Lysis Buffer to release plasmid DNA into the solution. Contaminants such as proteins and chromosomal DNA are precipitated by the neutralization buffer. The supernatant, which contains the plasmid DNA, binds to the magnetic particles, and a subsequent wash with an alcohol-containing buffer effectively removes impurities, resulting in purified plasmid DNA.
  • This kit is compatible with the MPure-32™ or MPure-96™ aNAP System, or other compatible high-throughput instruments, facilitating a faster extraction process that takes approximately 40 - 50 minutes.
TRUSTED_SUSTAINABILITY

Specifications

Form Liquid
Quantity 50 Preps
Type Magnetic Affinity Bead
Sufficient For Plasmid
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