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R&D Systems™ MagCellect Mouse Feeder Cell Removal Kit

Catalog No. MAGM215
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Description

The MagCellect™ Mouse Feeder Cell Removal Kit is designed to isolate stem cells via a negative selection principle. The resulting cell preparation is highly enriched with stem cells. Typical purity of recovered stem cells ranged between 90-97%.

A mononuclear cell suspension is first incubated with the Mouse Feeder Cell Removal Biotinylated Antibody that targets the unwanted cells. Streptavidin (SA) Ferrofluid is added to the reaction that allows the SA coated nanoparticles to interact with the biotinylated antibody tagged cells. The tube containing the cell suspension is then placed within a magnetic field. Magnetically tagged cells will migrate toward the magnet (unwanted cell fraction), leaving the untagged cells or desired cell population in suspension to be harvested by aspiration while the tube remains in the magnetic field. The enriched cell preparation is then available for a variety of applications including tissue culture, immune status monitoring and flow cytometry.

Kit Contents:

  • Mouse Feeder Cell Removal Biotinylated Antibody (0.5 mL of a phosphate buffered solution containing BSA and preservative)
  • Streptavidin Ferrofluid (2.0 mL of a solution containing BSA and preservatives)
  • MagCellect™ Plus (10X) Buffer (50 mL of a 10X concentrated buffer)

Specifications

Content And Storage Store the unopened product at 2°C to 8°C. Do not use past expiration date.
Quantity 1 Kit
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