Applied Biosystems™ MagMAX™ Pro HT NoSpin Plasmid MiniPrep Kit

Catalog No.	No. of Reactions
A58309	96 Reactions
A58310	384 Reactions
A58311	1152 Reactions
A58312	2304 Reactions

Catalog No. A58309 Supplier Applied Biosystems™ Supplier No. A58309

Description

The MagMAX Pro HT NoSpin Plasmid Miniprep Kit enables scalable, ultra-rapid purification of high-quality, transfection-grade plasmid DNA (pDNA) from bacterial cultures. Using magnetic-bead technology, it simplifies workflows by eliminating centrifugation and vacuum filtration bottlenecks.

The Applied Biosystems MagMAX Pro HT NoSpin Plasmid Miniprep Kit provides a scalable and ultra-rapid approach for purifying high quality, transfection-grade plasmid DNA (pDNA) directly from bacterial culture. Utilizing advanced magnetic-bead technology, the MagMAX Pro HT NoSpin kit drastically simplifies and streamlines plasmid purification workflows by eliminating centrifugation and vacuum filtration bottlenecks.

MagMAX Pro HT NoSpin Plasmid Miniprep Kit features include:

• Bead-based—utilizes dual para-magnetic beads for plasmid isolation without time-intensive processing steps (centrifugation, vacuum filtration)
• Automation enabled—turn-key, automation-friendly protocols and scripts, allowing 96 samples to be processed in under 35 minutes on KingFisher Apex and KingFisher Flex systems
• Fast plasmid DNA isolation—35 minutes from start to completion and ready for downstream applications
• Wide range of compatibility—typical E. coli cultures, common medias, and range of plasmids all acceptable
• High-quality pDNA yields of up to 20 μg, ready for downstream applications
• Ready for transfection—isolated pDNA with very low endotoxin levels < 1 EU/μg

Specifically designed for high-throughput plasmid isolation, the MagMAX Pro HT NoSpin technology is automation friendly and suited for use with KingFisher Apex, KingFisher Flex, and KingFisher Presto purification systems, as well as integrated liquid handling robots.

The MagMAX Pro HT purification chemistry is both effective and ecofriendly. Kits include a novel endotoxin reduction buffer to remove pyrogenic contaminates, and buffers are free of ethanol and chaotropic salts.

pDNA purified with the MagMAX Pro HT NoSpin kit is suitable for a wide array of downstream applications, such as transfection, cloning, enzymatic restriction, in vitro transcription, and sequencing.

Specifications

• Content And Storage: 2°C to 8°C BactBind Beads, PlasmidPure Beads, Endotoxin Reduction Buffer
  15°C to 25°C RNAse A, Resuspension Buffer, Lysis Buffer, Precipitation Buffer, Wash 1 Buffer, Wash 2 Buffer, Elution Buffer
• Format: 96 Deep-well Plate
• Isolation Technology: Magnetic Bead
• Scale: Mini
• Sample Type: Bacteria, E. coli
• Elution Volume: 100 μL
• For Use With (Equipment): KingFisher Apex, Flex and Presto Processors with 96 Deep Well Heads, Liquid Handling Robotic Systems
• For Use With (Application): Cloning, Transfection, Protein Expression, Protein Production, mRNA production via In Vitro Transcription, Gene Editing, Sequencing, PCR, Nucleic acid labeling, PCR
• No. of Reactions: 96 Reactions
• Nucleic Acid Type: Plasmid DNA (pDNA)
• Plate Compatibility: KingFisher 96 Deep Well Plates with or without barcodes
• Product Type: Automated, pDNA extraction kit
• Quantity: 1 Ea.
• Purity: Transfection Grade
• Sample Volume: Bacterial Culture, 1 mL per well
• Test Time: ~ 32 min. for 96 samples, 1 KingFisher 96 DWP
• Yield: Up to 20 μg pDNA using high copy plasmid
• Size Distribution: 5-12kb

Frequently Asked Questions (FAQs)

Can I grow an overnight bacterial culture directly in the KingFisher 96-well plate when using the MagMAX Pro HT NoSpin Plasmid MiniPrep Kit?

Yes, the KingFisher 96-well plate can be used for overnight bacterial culture growth with the MagMAX Pro HT NoSpin Plasmid MiniPrep Kit. However, growth conditions depend on factors such as media type, host strain, plasmid copy number, and plasmid size. For best results, optimize orbital shaker settings and monitor OD600 to minimize well-to-well variation and ensure consistent plasmid yield.

How does plasmid copy number affect yields with the MagMAX™ Pro HT NoSpin Plasmid MiniPrep Kit?

High-copy plasmids yield higher amounts of DNA and are ideal for most downstream applications. Low-copy plasmids can be extracted, but yields may be lower and may not be sufficient for all transfection protocols. For low-copy plasmids, optimizing culture conditions, increasing culture volume, or performing multiple elution steps may help improve yield.

Which bacterial strains are compatible with the MagMAX Pro HT NoSpin Plasmid MiniPrep Kit?

The MagMAX Pro HT NoSpin Plasmid MiniPrep Kit is compatible with K12 E. coli derivatives, including DH5alpha, TOP10, and DH10B strains. Plasmid yield may vary with low-copy origins, strong host dependencies, or high genomic integration tendencies.

Should I be concerned about bead clumps in the BactBind Beads or PlasmidPure Beads bottles before use?

No, bead settling during transit is normal. This does not affect performance. We recommend vortexing well, before use.

What should I do if there are precipitates in the Lysis Buffer within the MagMAX Pro HT NoSpin Plasmid MiniPrep Kit?

We recommend warming the buffer at 30-37 degrees C for 30 min, and then inverting to mix until the precipitates dissolve.