Applied Biosystems™ MagMAX™ Pure Bind Beads

Catalog No.	Quantity
A58522	50 mL
A58521	5 mL
A58523	250 mL

Catalog No. A58522 Supplier Applied Biosystems™ Supplier No. A58522

Description

MagMAX Pure Bind Beads are ready-to-use magnetic beads that provide optimized size selection and PCR cleanup, removing small fragments such as dNTPs, salts, primers, and primer-dimers.

MagMAX Pure Bind Beads are ready-to-use magnetic beads that provide optimized PCR cleanup, removing small fragments such as dNTPs, salts, primers, and primer-dimers. The magnetic bead technology allows for consistent binding capacity and highly reproducible results. The cleanup protocol is optimized for DNA fragments larger than 90 bp.

MagMAX Pure Bind Beads can seamlessly integrate into established workflows using magnetic beads for PCR cleanup. They can be used to clean up impurities throughout next-generation sequencing workflows.

Features of MagMAX Pure Bind Beads include:

• >90% recovery of DNA fragments larger than 90 bp
• Flexibility of starting sample volumes with the ability to scale
• Consistent results
• Compatible with manual and automated processes
• Room temperature storage

The use of MagMAX Pure Bind Beads does not require multiple bottles or long protocols. This product consists of just one bottle containing the optimized magnetic bead formulation, allowing a seamless transition into established workflows.

Sustainability
Additionally, MagMAX Pure Bind Beads are considered a more sustainable/greener product that can be shipped and stored at room temperature, allowing a grab-and-go effect to cut down on lab time. With the use of magnetic beads, the protocol can be completed manually or on automated systems, like KingFisher instruments.

Increase efficiency with compatible workflow products

KingFisher automated purification systems:
MagMAX Pure Bind Beads have been optimized for use on KingFisher automated purification systems. Choose from the KingFisher Apex (Cat. No. 5400910) and KingFisher Flex (Cat. No. 5400610) systems to streamline your DNA cleanup. Reduce sample processing hands-on time while achieving excellent PCR cleanup quality and reproducible results. Users can process 60 μL of PCR sample volume in high-throughput KingFisher 96 deep-well plates. BindIx and BindIt software scripts for KingFisher Apex and Flex systems can be downloaded below.

Streptavidin beads for target sequence enrichment:
For NGS library construction within NGS workflows, MagMAX Pure Bind Beads can be used in conjunction with Dynabeads Streptavidin for Target Enrichment (Cat. No. 65605D) to increase library concentrations and enhance sensitivity.

Specifications

• GC-Rich PCR Performance: High
• For Use With (Application): NGS Cleanup, PCR Cleanup, PCR Purification, DNA Purification, Sequencing
• Quantity: 50 mL
• Test Time: 20 min.
• Form: Beads in Suspension

Frequently Asked Questions (FAQs)

Can I use MagMax Pure Bind Beads for cleanup of cycle sequencing reactions in Sanger Sequencing?

We do not recommend using MagMax Pure Bind Beads for cleanup of cycle sequencing reactions. Fragments below 90 bp will be lost, so a large part of the beginning of a sequence can not be sequenced following cleanup using MagMax Pure Bind beads.

How long can MagMAX Pure Bind beads be stored at ambient temperatures?

MagMAX Pure Bind beads are stable at ambient temperatures for 18 months post-production.

Are MagMAX Pure Bind beads compatible with automated workflows?

Yes, MagMAX Pure Bind beads can be used with KingFisher Apex, KingFisher Flex, and KingFisher Duo instruments.

Can I perform size selection with MagMAX Pure Bind beads?

Yes, size selection can be performed by adjusting the ratio of MagMAX Pure Bind beads and sample.

What is the smallest size fragment that can be recovered using MagMAX Pure Bind beads?

MagMAX Pure Bind beads are successful in recovering fragments down to 90 bp in size.

What is the expected recovery for MagMAX Pure Bind beads?

MagMAX Pure Bind beads are designed for purification of PCR products that are 90 bp or larger in size. The recovery ranges from 80-90%.

Can I use MagMAX Pure Bind beads to purify RNA?

MagMAX Pure Bind beads have been designed to bind to DNA and are not manufactured under RNase-free conditions. Therefore, the product has not been tested to be RNase-free and we do not recommend using it for RNA cleanup. We offer several RNA purification kits to choose from to suit your specific needs.

If my MagMAX Pure Bind beads were stored at 4 degrees C, can they still be used?

Yes, MagMAX Pure Bind beads can be stored at 4 degrees C and used if they are brought up to room temperature and mixed well before use.

Can I store MagMAX Pure Bind beads at ambient temperatures?

Yes, MagMAX Pure Bind beads were developed to be stored at ambient temperatures to provide a more sustainable solution to our customers and to allow for a grab-and-go workflow.