Applied Biosystems™ MagMAX™ Total Nucleic Acid Isolation Kit

Description

The MagMAX™ Total Nucleic Acid Isolation Kit is designed for the rapid, high-throuphput purification of RNA and genomic DNA from a variety of samples including viral, blood, and bacterial samples

The MagMAX™ Total Nucleic Acid Isolation Kit includes zirconia beads for the mechanical disruption of difficult pathogens. RNA purified using the kit is ideal for PCR and RT-PCR applications. The kit uses magnetic bead–based purification and includes sufficient reagents for 100 reactions.

• Complete, ready-to-use reagents for RNA and DNA purification from tough-to-lyse bacteria
• Highly effective sample homogenization using bead beating and chemical lysis
• Magnetic bead–based purification technology allows effective removal of PCR inhibitors
• Nucleic acid purification is easily scalable and suitable for automation

Proven with a Wide Range of Samples:

• Biological Samples
• Feces and whole blood
• Biofluids such as milk, urine, nasal fluids, swab samples, semen, sputum, and spent culture medium
• Bacterial cultures and mammalian cell cultures
• Environmental Samples
• River and pond water
• Agricultural samples such as manure pits and cow alleyways

Benefits of the MagMAX™ Total Nucleic Acid Isolation Kit:

The MagMAX™ Total Nucleic Acid Isolation Kit procedure combines an effective method sample disruption with magnetic bead–based nucleic acid isolation and purification technology. The MagMAX™ microspherical paramagnetic beads have a large available binding surface and can be fully dispersed in solution, allowing thorough nucleic acid binding, washing, and elution. This results in consistent yields of high-quality nucleic acid with >50% recovery and little sample-to-sample variation (recovery may vary depending on sample type). The entire procedure can be completed in approximately 1 hour.

Overview of the Procedure:

Samples are disrupted using zirconia beads in a guanidinium thiocyanate–based solution; this rapidly releases nucleic acid while simultaneously inactivating nucleases. Samples are then diluted with isopropanol, and paramagnetic beads are added to the sample. The beads bind nucleic acids and are then immobilized on magnets and washed to remove proteins and other contaminants. A second wash solution is used to remove residual binding solution. Finally, nucleic acid is eluted in a small volume of low-salt buffer. The MagMAX™ Total Nucleic Acid Isolation Kit procedure is designed for processing samples in 96-well plates, thus 96 samples can be processed at once; however, the kit can also be used with fewer than 96 samples.

Accessory Products:

Lysis/Binding Solution Concentrate (Cat. No. AM8500), Wash Solution 1 Concentrate (Cat. No. AM8504), and Wash Solution 2 Concentrate (Cat. No. AM8640) are components of the MagMAX™ Total Nucleic Acid Isolation Kit that may also be purchased separately.

Specifications

• Content And Storage:

  • Carrier NA and Lysis/Binding Enhancer, store at -20°C
  • RNA Binding Beads, 4°C
  • Wash Solution 2 Concentrate, 4°C or room temperature
  • Elution Buffer, 4°C or room temperature
  • Lysis/Binding Solution Concentrate, room temperature
  • Wash Solution 1 Concentrate, room temperature
  • Processing Plates, room temperature
  • Lids, room temperature
  • PBS Buffer, room temperature

• Format: 96-well Plate
• Isolation Technology: Magnetic Bead
• Sample Type: Bacteria, Blood, Cells, Liquid Samples (e.g. Serum)
• Final Product Type: RNA, DNA
• For Use With (Application): Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Southern Blotting, PCR, Northern Blotting
• High-throughput Compatibility: High-throughput Compatible
• Product Line: MagMAX™
• Quantity: 100 preps
• Target: Total RNA, Total Nucleic Acids, Genomic DNA
• Test Time: 1 hr.
• Yield: 420 ng
• Starting Material Amount: Up to 0.3 g solid samples or 175 μl liquid samples

Safety and Handling

WARNING: Cancer and Reproductive Harm - www.P65Warnings.ca.gov

For Veterinary Use Only.