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Thermo Scientific™ MagnaBind™ Streptavidin Beads

Catalog No. PI21344
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Quantity:
5 mL

Description

Enable benchtop magnetic affinity purification with these ligand-coated (streptavidin, Protein A and G, GAM IgG, GAR IgG) magnetic particles and magnet separators.

Thermo Scientific MagnaBind Streptavidin Beads are convenient for affinity purification or separation methods involving of biotin-labeled molecules. To remove the MagnaBind Beads from the suspension, an external magnetic field is used.

Features of MagnaBind Streptavidin Beads:

  • Composition: Silanized iron oxide
  • Magnetization: 25-35EMU/g
  • Type of Magnetization: Superparamagnetic (no magnetic memory)
  • Surface Area: >100m2/g
  • Bead Size: 1-4μm diameter
  • Settling Rate: 4% in 30 minutes
  • Effective Density: 2.5g/mL
  • Number of Beads: 1 x 108 beads/mg
  • pH Stability: Aqueous solution, above pH 4.0
  • Concentration: 5 mg/mL

Streptavidin is a 60kDa protein from Streptomycetes avidinii. The protein is a tetramer having four biotin-binding sites. Unlike avidin, streptvidin has a low isoelectric point (pI=5) and no carbohydrate groups, resulting in low nonspecific binding. The high-affinity interaction between streptavidin and biotin cannot be dissociated efficiently except with very harsh conditions, such as boiling in sample loading buffer for SDS-PAGE or 8M guanidine HCl, pH 1.5. Consequently, it is often possible to elute binding partners in an interaction complex without also eluting the biotinylated component.

Applications:

  • Cell sorting using either positive or negative selection
  • Protein purification or immunoassays using either direct or indirect methods
TRUSTED_SUSTAINABILITY

Specifications

Concentration Slurry: 5 mg/mL
Content And Storage Upon receipt store product at 4°C. Do not freeze product. Product is shipped at ambient temperature.
Description MagnaBind Streptavidin Beads
Color Brown
For Use With (Application) Affinity Purification
Product Type Streptavidin Magnetic Beads
Ligand Type Streptavidin
Product Line MagnaBind™
Quantity 5 mL
Volume (Metric) 5 mL
Diameter (Metric) 1 to 4 μm
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Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

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