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Invitrogen™ MagniSort™ Human CD4 T cell 2-Step Enrichment Kit
Description
The MagniSort™ Human CD4 2-Step Enrichment Kit is designed for the magnetic separation of CD4+ T cells by positive selection after first depleting monocytes. It has been optimized for the isolation of CD4+ T cells from human peripheral blood monocuclear cells, utilizing biotinylated Anti-Human CD14 and Anti-Human CD4 antibodies, as well as streptavidin-coated magnetic beads. In the first step, monocytes are bound by Anti-Human CD14 antibody and then magnetic beads. When placed in a magnetic field, the monocytes are held in the magnet while the remaining cells are decanted and used for the second step. In the second step, the monocyte-depleted cells are bound by Anti-Human CD4 antibody and then magnetic beads. Now, when placed in the magnetic field, the CD4+ T cells are held in the magnet while undesired cells can be decanted.
After enrichment, the purity of selected cells can be verified by staining with Anti-Human CD4, clones OKT4 or SK3.
Reported Application: Magnetic Cell Separation
Specifications
Specifications
| Cell Type | CD4+ |
| For Use With (Application) | Magnetic Cell Separation |
| Product Type | Cell Enrichment Kit |
| Format | Kit |
| No. of Tests | 200 tests |
| Quantity | 200 tests |
| Target Species | Human |
For Research Use Only. Not for use in diagnostic procedures.
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