Invitrogen™ MagniSort™ Human CD4 T cell 2-Step Enrichment Kit

Catalog No.	Quantity
88-026-83174	200 tests

Catalog No. 88-026-83174 Supplier Invitrogen™ Supplier No. 8802683174

Description

The MagniSort™ Human CD4 2-Step Enrichment Kit is designed for the magnetic separation of CD4+ T cells by positive selection after first depleting monocytes.

The MagniSort™ Human CD4 2-Step Enrichment Kit is designed for the magnetic separation of CD4+ T cells by positive selection after first depleting monocytes. It has been optimized for the isolation of CD4+ T cells from human peripheral blood monocuclear cells, utilizing biotinylated Anti-Human CD14 and Anti-Human CD4 antibodies, as well as streptavidin-coated magnetic beads. In the first step, monocytes are bound by Anti-Human CD14 antibody and then magnetic beads. When placed in a magnetic field, the monocytes are held in the magnet while the remaining cells are decanted and used for the second step. In the second step, the monocyte-depleted cells are bound by Anti-Human CD4 antibody and then magnetic beads. Now, when placed in the magnetic field, the CD4+ T cells are held in the magnet while undesired cells can be decanted.

After enrichment, the purity of selected cells can be verified by staining with Anti-Human CD4, clones OKT4 or SK3.

Reported Application: Magnetic Cell Separation

Specifications

• Cell Type: CD4+
• For Use With (Application): Magnetic Cell Separation
• Product Type: Cell Enrichment Kit
• Format: Kit
• No. of Tests: 200 tests
• Quantity: 200 tests
• Target Species: Human

For Research Use Only. Not for use in diagnostic procedures.