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IBA LifeSciences MagStrep type3 Strep-Tactin beads (2ml) are magnetic beads (5% suspension) to purify biotinylated proteins
Supplier: IBA LifeSciences 21613002
MagStrep “type3” Strep-Tactin beads have an agarose surface coated with the streptavidin variant Strep-Tactin and intended for purification of biotinylated proteins. Originally, Strep-Tactin was developed for binding and purification of Strep-tagII/Twin-Strep-tag proteins. Strep-Tactin still has the capability to bind biotin, which is used as specific competitor for elution of Strep-tagged proteins. The release is carried out under mild and physiological conditions by adding an excess of free biotin. This reversible binding to Strep-Tactin make them to a perfect tool for isolation of biotinylated proteins or complexes via pull-downs or co-immunoprecipitation (Co-IP) in batch format or high-throughput screenings.Due to the magnetic core no centrifugation step is required since the beads can be easily separated with a magnetic separator, reducing the risk of disturbing the pellet or loss of beads within the wash step to zero. 20 μl suspension correspond to 1 μl magnetic beads.
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