Invitrogen™ Human MAP3K3 (MEKK3), GST Tag Recombinant Protein

Catalog No.	Quantity
PV3876	10 μg

Catalog No. PV3876 Supplier Invitrogen™ Supplier No. PV3876

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Protein Form: Full Length.

MAP3K3 (MEKK3) directly regulates MAPK8 and extracellular signal-regulated protein kinase (ERK) pathways by phosphorylating multiple MAP2Ks. Northern blot analysis revealed a 4. 6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Specifications

• Accession Number: Q99759
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50mM tris with 0.5mM EDTA, 50% glycerol, 0.02% Triton X-100, 150mM NaCl, 2mM DTT and no preservative; pH 7.5
• Gene ID (Entrez): 4215
• Molecular Weight (g/mol): 99.8 kDa
• Name: Human MAP3K3 (MEKK3), GST Tag
• pH Range: 7.5
• Purification Method: Purified
• Quantity: 10 μg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: AW548911; MAP/ERK kinase kinase 3; MAP3K3; MAPK/ERK kinase kinase 3; MAPKKK3; MEK kinase 3; MEKK 3; Mekk3; mitogen activated protein kinase kinase kinase 3; mitogen-activated protein kinase kinase kinase 3; mKIAA4031
• Common Name: MEKK3
• Gene Symbol: Map3k3
• Kinase Family: STE11 Kinase Family
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: GST-tag
• Sequence: Full length
• Content And Storage: Storage Buffer: 50 mM Tris, pH 7.5, 150 mM NaCl, 0.5 mM EDTA, 0.02% Triton X-100, 2 mM DTT, 50% glycerol
  Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws.
• Assay Entry: Biochemical terbium phospho-specific activity, Biochemical binding/kinetic
• Mass: 10 μg
• Kinase Group: Ser⁄Thr Kinases: STE Group
• Shipping Condition: Dry Ice
• System Compatibility: LanthaScreen™ Antibody, LanthaScreen™ Substrate, Omnia™ Bulk Substrate
• Tag Position: N-Terminal
• Target Entry: MAP3K3
• Target Gene: mitogen-activated protein kinase kinase kinase 3
• Expression System: Insect cells
• Protein Family: Kinases & Inhibitors
• Protein Form: Recombinant, Full Length
• Form: Liquid
• Protein Subtype: Serine/Threonine Kinases
• Research Category: Drug Discovery: Signal Transduction
• Purity or Quality Grade: 60% by SDS-PAGE