Invitrogen™ Human MAP4K4 (HGK), GST Tag Recombinant Protein

Catalog No. PV3687

Supplier: Invitrogen™ PV3687

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Protein Form: Recombinant, Catalytic Domain.

MAP4K4 (HGK) is a serine/threonine kinase which plays an important role in stress-activated MAPK core signaling pathways, the response to environmental stress and cytokines such as TNF-alpha. It appears to act upstream of the JUN N-terminal pathway. This protein is thought to interact with the SH3 domain of the adapter proteins Nck. HGK binds, via its CNH regulatory domain, to the N-terminal region of SPG3A. Expression appears to be ubiquitous, expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain, and isoform 4 is predominant in the liver, skeletal muscle and placenta.

Specifications

• Accession Number: O95819
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50mM tris with 50% glycerol, 2mM DTT, 0.02% Triton X-100, 0.5mM EDTA, 150mM NaCl and no preservative; pH 7.5
• Gene ID (Entrez): 9448
• Molecular Weight (g/mol): 65 kDa
• Name: Human MAP4K4 (HGK), GST Tag
• pH Range: 7.5
• Purification Method: Purified
• Quantity: 10 μg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: 9430080K19Rik; AU043147; AU045934; AW046177; epididymis secretory protein Li 31; FLH21957; FLJ10410; FLJ20373; FLJ90111; HEL-S-31; hepatocyte progenitor kinase-like/germinal center kinase-like kinase; HGK; HPK/GCK-like kinase HGK; KIAA0687; Map4k4; MAPK/ERK kinase kinase kinase 4; MEK kinase kinase 4; MEKKK 4; MEKKK4; mitogen activated protein kinase kinase kinase kinase 4; mitogen-activated protein kinase kinase kinase kinase 4; NCK interacting kinase; nck-interacting kinase; NIK; Ste20 group protein kinase HGK
• Common Name: HGK
• Gene Symbol: MAP4K4
• Protein Length: 1-328
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: GST-tag
• Sequence: 1-328
• Content And Storage: -80°C, Avoid Freeze/Thaw Cycles
• Assay Entry: Biochemical phospho-specific activity (no antibody), Biochemical terbium phospho-specific activity, Biochemical binding/kinetic
• Kinase Group: Ser⁄Thr Kinases: STE Group
• Shipping Condition: Dry Ice
• System Compatibility: LanthaScreen™ Antibody, LanthaScreen™ Substrate, Zin-LYTE™, Omnia™ Bulk Substrate
• Target Entry: MAP4K4
• Expression System: Insect cells
• Protein Family: Kinases & Inhibitors
• Protein Form: Recombinant, Catalytic Domain
• Form: Liquid
• Protein Subtype: Serine/Threonine Kinases
• Research Category: Drug Discovery: Signal Transduction