Invitrogen™ Multispecies MAPK Family Activation InstantOne™ ELISA Kit

Catalog No.	Quantity
85-861-9511	96 Tests

Catalog No. 85-861-9511 Supplier Invitrogen™ Supplier No. 858619511

Description

ELISA

The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated ERK 1/2 (human, mouse, hamster), p38 MAPK (human, mouse), and JNK 1/2 (human, mouse) in cell lysates. Detection of these phosphorylated molecules in rat is also expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.

MAP kinases play a significant role in many biological processes, including cell adhesion and spreading, cell differentiation and apoptosis. MAPKBP-1 (mitogen-activated protein kinase binding protein 1), also known as JNKBP-1, is a 1,514 amino acid protein that contains twelve WD repeats. Induced by TRAF2 (TNF receptor-associated factor 2) and Tak1 (TGF-β-activated kinase 1), MAPKBP-1 is thought to act an adaptor protein for NFκB (nuclear factor κ-B) activation. MAPKBP-1 interacts with JNK3 and may promote TRAF2 polyubiquitination. MAPKBP-1 exists as six alternatively spliced variants and is encoded by a gene located on human chromosome 15. Human chromosome 15 houses over 700 genes and comprises nearly 3% of the human genome. Angelman syndrome, Prader-Willi syndrome, Tay-Sachs disease and Marfan syndrome are all associated with defects in chromosome 15-localized genes.

Specifications

• Accession Number: P27361, P28482, Q16539
• Content And Storage: • User Manual
  • phospho-ERK 1/2 (Thr202/Tyr204, Thr182/Tyr187) Capture Antibody Reagent, 1 mL
  • phospho-ERK 1/2 (Thr202/Tyr204, Thr182/Tyr187) Detection Antibody Reagent, 1 mL
  • phospho-p38 MAPK (Thr180/Tyr182) Capture Antibody Reagent, 1 mL
  • phospho-p38 MAPK (Thr180/Tyr182) Detection Antibody Reagent, 1 mL
  • phospho-JNK 1/2 (Thr183/Tyr185) Capture Antibody Reagent, 1 mL
  • phospho-JNK 1/2 (Thr183/Tyr185) Detection Antibody Reagent, 1 mL
  • InstantOne Assay Plate, 96 well (sealed)
  • Cell Lysis Buffer (5X), 10 mL
  • Enhancer Solution, 1 mL
  • Wash Buffer (10X), 15 mL
  • Detection Reagent (TMB Substrate), 12 mL
  • Stop Solution, 12 mL
  • Positive Control Cell Lysate lyophilized, 0.25 mL
  • Plate seal, 1 ea
• Conjugate: HRP
• Immunoassay Kit Format: Coated ELISA Kit
• Product Type: ELISA
• Sample Type: Cell Lysates
• Target Species: Human, Mouse
• For Use With (Application): ELISA
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: MAPKMAPK; ERK; ERK-2; ERK2; ERT1; MAPK2; P42MAPK; PRKM1; PRKM2; p38; p40; p41; p41mapk; p42-MAPK;ERK-1; ERK1; ERT2; HS44KDAP; HUMKER1A; P44ERK1; P44MAPK; PRKM3; p44-ERK1; p44-MAPK;JNK; JNK-46; JNK1; JNK1A2; JNK21B1/2; PRKM8; SAPK1; SAPK1c;CSBP; CSBP1; CSBP2; CSPB1;
• Gene ID (Entrez): 1432, 5594, 5595
• Gene Symbol: MAPK1, MAPK14, MAPK3
• Kit Contents: Capture Antibody, Detection Antibody Reagent, InstantOneTM Assay Plate, Cell Lysis Buffer, Enhancer Solution, Wash Buffer (10X), Substrate Reagent (TMB), Stop Solution, Positive Control Cell Lysate (lyophilized), Plate seal, Detection Antibody Reagent, InstantOne™ Assay Plate, Cell Lysis Buffer, Enhancer Solution, Wash Buffer (10X), Substrate Reagent (TMB), Stop Solution, Positive Control Cell Lysate (lyophilized), Plate seal
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 20 μL
• Storage Requirements: 2-8°C
• Synonym: ERK, p38, JNK
• Target: JNK1/JNK2/JNK3/ERK1/2/p38 MAPK alpha
• Test Time: 1 hr 20 min
• Total Assay Time: 1 hr 10 min