Invitrogen™ Human MBL Oligomer ELISA Kit

Catalog No.	Quantity
BMS23292	96 Tests

Catalog No. BMS23292 Supplier Invitrogen™ Supplier No. BMS23292

Description

ELISA

The Human Mannose Binding Lectin (Hu MBL-2) ELISA quantitates Hu MBL-2 in human serum or plasma. The assay will exclusively recognize both natural and recombinant Hu MBL-2. Principle of the method The Human MBL-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

MBL2 is a calcium-dependent lectin involved in innate immune defense. It binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. It also binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. MBL2 may bind DNA.

Specifications

• Accession Number: P11226
• Assay Range: 0.05-40 ng/mL
• Assay Sensitivity: 102 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Lectin
• Sample Type: Plasma, Serum
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: 27 kDa mannan-binding protein monomeric subunit; Ab2-001; Ab2-011; cMBl; COLEC1; collectin; Collectin1; collectin-1; c-type lectin; HSMBPC; L-MBP; mannan-binding lectin; mannan-binding lectin C; mannan-binding protein; mannose binding lectin (C); mannose binding lectin 2; mannose binding lectin 2 (protein C); mannose binding lectin, liver (A); mannose binding lectin, liver (C); mannose-binding lectin; mannose-binding lectin (protein C) 2; mannose-binding lectin (protein C) 2, soluble; mannose-binding lectin (protein C) 2, soluble (opsonic defect); mannose-binding lectin 2, soluble (opsonic defect); mannose-binding lectin C; mannose-binding lectin precursor protein; mannose-binding lectin-C; mannose-binding protein; Mannose-binding protein C; mannose-binding protein C (liver); mbl; MBL2; MBL2D; MBL-C; MBP; MBP1; MBPC; MBP-C; MBPD; MGC116832; MGC116833; pMBP-27; RA-reactive factor P28A subunit; ra-reactive factor polysaccharide-binding component p28A; RaRF p28A; RARF/P28A; serum protein; MBL; soluble mannose-binding lectin27 kDa mannan-binding protein monomeric subunit; Ab2-001; Ab2-011; cMBl; COLEC1; collectin; Collectin1; collectin-1; c-type lectin; HSMBPC; L-MBP; mannan-binding lectin; mannan-binding lectin C; mannan-binding protein; mannose binding lectin (C); mannose binding lectin 2; mannose binding lectin 2 (protein C); mannose binding lectin, liver (A); mannose binding lectin, liver (C); mannose-binding lectin; mannose-binding lectin (protein C) 2; mannose-binding lectin (protein C) 2, soluble; mannose-binding lectin (protein C) 2, soluble (opsonic defect); mannose-binding lectin 2, soluble (opsonic defect); mannose-binding lectin C; mannose-binding lectin precursor protein; mannose-binding lectin-C; mannose-binding protein; Mannose-binding protein C; mannose-binding protein C (liver); mbl; MBL2; MBL2D; MBL-C; MBP; MBP1; MBPC; MBP-C; MBPD; MGC116832; MGC116833; pMBP-27; RA-reactive factor P28A subunit; ra-reactive factor polysaccharide-binding component p28A; RaRF p28A; RARF/P28A; serum protein; MBL; soluble mannose-binding lectin; COLEC1; HSMBPC; MBL; MBL2; MBL2D; MBP; MBP-C; MBP1; MBPD
• Gene ID (Entrez): 4153
• Gene Symbol: MBL2
• Interassay CV: <10%
• Intraassay CV: <6%
• Kit Contents: Pre-coated 96 well plate, Standard (2 vials), HRP Detection Antibody, Assay buffer Concentrate, Wash buffer concentrate, TMB, Stop solution, Adhesive Plate Covers, Standard (2 vials), HRP Detection Antibody, Assay buffer Concentrate, Wash buffer concentrate, TMB, Stop solution, Adhesive Plate Covers
• Label or Dye: HRP
• Protein Aliases: Collectin-1, mannan-binding lectin, Mannan-binding protein, Mannose-binding lectin, mannose-binding lectin (protein C) 2, soluble (opsonic defect), mannose-binding lectin 2, Mannose-binding protein C, MBL2, MBP-C, MBP1
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 10 μL, Serum 10 μL
• Shipping Condition: Wet Ice
• Storage Requirements: 2-8°C
• Target: MBL2
• Test Time: 1 hr 20 min
• Total Assay Time: 3 hr 15 min