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Enzo Life Sciences Mca-RPPGFSAFK(Dnp) (1 mg)
Supplier: Enzo Life Sciences BMLP2270001
Protease substrate. Fluorogenic substrate for ECE-1 and ECE-2, ACE1 and ACE2, neprilysin, MMP-2 and MMP-9, thimet oligopeptidase, as well as cathepsin A, cathepsin X/Z, BACE, and insulinase, but not MMP-1.Mca fluorescence is quenched by the Dnp group until cleavage separates them. Ex.: 328nm, Em.: 393nm, although the following Ex/Em have also been used: 320-340/400-420. This substrate is useful for inhibitor screening and kinetic analysis. MW: 1388.5. Formulation: Lyophilized. Purity: ≥98% (HPLC). Sequence Mca-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys(Dnp)-OH[Mca=(7-methoxycoumarin-4-yl)acetyl;Dnp=2,4-dinitrophenyl]. Appearance: White to off-white solid. Solubility: Soluble in DMSO to at least 10mM. Long Term Storage: -20°C. Use/Stability: Store, as supplied, at -20°C for up to 1 year. Store solutions at -20°C for up to 3 months. Handling: Protect from light and moisture.
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