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Gibco™ MCDB 131 Medium, no glutamine
GSA_VA

MCDB 131 Medium, no glutamine

Manufacturer:  Gibco™ 10372019

Encompass

Catalog No. 10372019


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Description

Description

MCDB 131 Medium was originally developed by Knedler and Ham as reduced serum-supplemented medium for the culture of human microvascular endothelial cells (HUVEC). MCDB 131 Medium is also used with other cell types, including human omental microvascular cells, hepatocytes, myocytes, and smooth muscle cells.

Using MCDB 131
MCDB 131 Medium contains many components not found in traditional basal media, such as trace elements, putrescine, adenine, thymidine, and higher levels of some amino acids and vitamins. These additions allow the medium to be supplemented with very low levels of serum or defined components. MCDB 131 Medium contains no proteins or growth factors, and is often supplemented with EGF, hydrocortisone, glutamine, and low levels of Fetal Bovine Serum (FBS). The FBS concentration must be optimized for each cell type. MCDB 131 Medium uses a sodium bicarbonate buffer system (1.176 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Dual-site cGMP manufacturing
For supply chain continuity, we manufacture MCDB 131 at two separate facilities, located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP-manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.

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Specifications

Specifications

1X
Sterile-filtered
Room Temperature
Gibco™
No Glutamine
12 Months From Date of Manufacture
human microvascular endothelial cells (HMVEC), human omental microvascular cells, hepatocytes, myocytes, smooth muscle cells
HEPES, L-Glutamine
Low Glucose
No HEPES
MCDB 131 Medium
Storage conditions: 2–8°C (protect from light)
Shelf life: 12 months from date of manufacture

500 mL
Liquid
Phenol Red
Sustainable packaging
Phenol Red, Sodium Pyruvate
Mammalian Cell Culture
Sodium Pyruvate
SDS
Documents
Product Certifications
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