Mebiol is a thermoreversible hydrogel that gels at 37C and transitions back to a liquid at 4C. Mebiol is also biologically inert, allowing for easy spheroid formation in the hydrogel phase, and easy cell retrieval during the cold-melt phase. Mebiol Thermoreversible Hydrogel is 1 gram of a copolymer of poly(N-isopropylacrylamide) and poly(ethylene glycol) (PNIPAAm-PEG). The defining feature of Mebiol is its temperature reversible sol-gel transition. Chill Mebiol to turn it into a sol (meaning it handles like a liquid). Heat Mebiol to turn it into a solid hydrogel. The unique thermoreversible feature allows for extremely easy cell handling. Mebiol is easy to use, non-toxic, 100% synthetic and has high transparency. Published applications include stem cell and pluripotent stem cell culture, expansion, and differentiation, spheroid culture, cell implantation, organ and tissue regeneration, drug delivery and more.