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Gibco™ Human Mammary Epithelial Cell Basal Medium (formerly Medium 171)
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Catalog No. M171500
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M171500 500 mL
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Catalog No. M171500 Supplier Gibco™ Supplier No. M171500
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Description

A sterile, liquid tissue culture media that is intended for use in a complete culture environment for the growth of normal human mammary epithelial cells. Contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals, and inorganic salts.

Human Mammary Epithelial Cell Basal Medium (formerly 'Medium 171') is a sterile, liquid tissue culture medium intended for use in a complete culture environment for the growth of normal human mammary epithelial cells. This medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals, and inorganic salts. It does not contain antibiotics, antimycotics, hormones, growth factors, or proteins. It is HEPES and bicarbonate buffered and designed for use in an incubator with an atmosphere of 5% CO2/95% air.

To support plating and long-term proliferation of normal human mammary epithelial cells, Human Mammary Epithelial Cell Basal Medium must be supplemented with Mammary Epithelial Growth Supplement (MEGS) (Cat. No. S0155). Each 5-mL bottle of MEGS contains all of the growth factors, hormones, and tissue extracts necessary for the culture of normal human mammary epithelial cells and is the correct amount of supplement for a 500 mL bottle of Human Mammary Epithelial Cell Basal Medium.

When a 500 mL bottle of medium is supplemented with MEGS, the final concentrations of the MEGS components in the supplemented medium are: BPE, 0.4% v/v; recombinant human insulin-like growth factor-I, 0.01 μg/mL; hydrocortisone, 0.5 μg/mL; and recombinant human epidermal growth factor, 3 ng/mL.

TRUSTED_SUSTAINABILITY

Specifications

Cell Type Mammalian Cells
Classification Serum-free
Form Liquid
Product Type Medium 171
Serum Level Serum-free
Sterility Sterile-filtered
Sterilization Method Sterile-filtered
With Additives Phenol Red
Product Line Cascade Biologics
Quantity 500 mL
Shipping Condition Room Temperature
Content And Storage 4 degrees C
Culture Type Mammalian Cell Culture
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Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

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