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Gibco™ Medium 199 (2X)
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Catalog No. 21157029
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21-157-029 250 mL
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Description

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells.

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco™ Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This Medium 199 is modified as follows:

With: L-glutamine

Without: HEPES, Phenol Red

The complete formulation is available.

Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2. This Medium 199 is made with Earle's salts.

Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

TRUSTED_SUSTAINABILITY

Specifications

Cell Type Mammalian Cells
Concentration 2 X
Form Liquid
Product Type Medium 199
Sterility Sterile-filtered
With Additives Glutamine, Earle's Balanced Salt Solution (EBSS)
Without Additives No HEPES, No Phenol Red
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Product Line Gibco
Quantity 250 mL
Shipping Condition Room Temperature
Content And Storage Storage conditions: 2 – 8°C. Protect from light.
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Culture Type Mammalian Cell Culture
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For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

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