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ATCC MEF (C57BL/6); Mouse fibroblast
Supplier: ATCC SCRC1008
MEF (C57BL/6) [MEF-BL/6-1] is a fibroblast cell that was isolated from the stroma of a mouse. This cell can be used as a feeder layer to support the growth of embryonic stem (ES) cells and for the maintenance of ES cells in the undifferentiated state. The growth of these cells must be arrested before they can be used as a feeder layer. ATCC has successfully treated the cells with mitomycin C for use as a feeder layer. If the MEFs are being used as a feeder layer for ES cells, it is not recommended to use them past passage no. 6 (P6).
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