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  5. Invitrogen™ MEGAscript™ SP6 Transcription Kit 

Invitrogen™ MEGAscript™ SP6 Transcription Kit

Catalog No. AM1330
Encompass_Preferred
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Product Type:
SP6 In Vitro Transcription kit
Quantity:
40 Reactions

Description

Synthesize 10 to 50 times the amount of RNA produced by conventional transcription reactions

Novel transcription reaction conditions and Ambion's patented, high-yield technology allow the synthesis of 10 to 50 times the amount of RNA produced by conventional transcription reactions. Each Ambion kit includes sufficient reagents for 40 reactions.

  • Exclusive, ultrahigh yield technology is fast: 2 hour reaction time
  • Amplifies aRNA for gene array analysis and other applications
  • Efficiently incorporates many modified nucleotides
  • A typical 20μL MEGAscript reaction with 1μg of the pTRI-Xef-1α control template will yield over 100μg of transcript
  • Versatility of the MEGAscript kit allows for manipulation of the reactions to include specialized reagents such as modified nucleotides, cap analog, or additional polymerase
  • Accessory Products:
  • MessageAmp™ aRNA Amplification Kits are powered by MEGAscript technology and have been developed especially for the amplification of cRNA prior to array analysis. To purify in vitro transcription products from free nucleotides, buffer components, and enzymes, use MEGAclear™ Kit (Mfr. No. AM1908) and the MEGAclear-96 Kit (Mfr. No. AM1909).

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage SP6 Enzyme Mix, 10X Reaction Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
Final Product Type Synthetic mRNA, mRNA
Format Kit
Sample Type mRNA, Synthetic mRNA
For Use With (Application) In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
No. of Reactions 40 Reactions
Product Line Ambion™, MEGAscript™
Product Type SP6 In Vitro Transcription kit
Quantity 40 Reactions
Promoter SP6
Shipping Condition Dry Ice
Yield 100 μg
Starting Material PCR Products, Linearized DNA plasmid
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For Research Use Only. Not for use in diagnostic procedures.

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