Invitrogen™ Human MELK, GST Tag Recombinant Protein

Catalog No.	Quantity
PV4823	10 μg

Catalog No. PV4823 Supplier Invitrogen™ Supplier No. PV4823

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Protein Form: Catalytic.

MELK is a serine/threonione kinase which is related to the AMPK family that has been linked to the cell cycle. MELK has also been shown to be overexpressed in cancer and has been identified has a potential target for cancer therapeutics. MELK has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis.

Specifications

• Accession Number: Q14680
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50mM tris with 150mM NaCl, 0.5mM EDTA, 2mM DTT, 0.02% Triton X-100, 50% glycerol and no preservative; pH 7.5
• Gene ID (Entrez): 9833
• Molecular Weight (g/mol): 66.8 kDa
• Name: Human MELK, GST Tag
• pH Range: 7.5
• Purification Method: Purified
• Quantity: 10 μg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: AI327312; hMELK; HPK38; Kiaa0175; Maternal embryonic leucine zipper kinase; Melk; mKIAA0175; MPK38; OTTHUMP00000046113; pEg3 kinase; Pk38; Protein kinase Eg3; protein kinase PK38; RP11-8N6.1; Tyrosine-protein kinase MELK
• Common Name: MELK
• Gene Symbol: MELK
• Kinase Family: CAMLK Kinase Family, CAMKL Kinase Family
• Protein Length: aa 1-340
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: GST-tag
• Sequence: 1-340
• Content And Storage: Storage Buffer: 50 mM Tris, pH 7.5, 50% glycerol, 0.5 mM EDTA, 0.02% Triton X-100, 150 mM NaCl, 2 mM DTT
  Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws.
• Assay Entry: Biochemical binding/kinetic, Biochemical phospho-specific activity (no antibody)
• Kinase Group: Ser⁄Thr Kinases: CAMK Group
• Shipping Condition: Dry Ice
• System Compatibility: Zin-LYTE™
• Target Entry: MELK
• Target Gene: maternal embryonic leucine zipper kinase
• Expression System: Insect cells
• Protein Family: Kinases & Inhibitors
• Protein Form: Recombinant, Catalytic
• Form: Liquid
• Protein Subtype: Serine/Threonine Kinases
• Research Category: Drug Discovery: Oncology, Signal Transduction
• Purity or Quality Grade: 95% by SDS-PAGE