Thermo Scientific™ Melon™ Gel IgG Purification Kit

Rapidly purify human, mouse and rabbit IgG from serum and other sources with this simpler alternative to traditional Protein A and Protein G methods.

Supplier:  Thermo Scientific™ 45212

Catalog No. PI45212

Description

Thermo Scientific™ Melon Gel IgG Purification Resins and Kits comprise a simpler and faster alternative to traditional Protein A and Protein G methods for purification of human, mouse and rabbit IgG from serum and other sources.

Melon Gel IgG Purification Kits enable fast, easy and gentle purification of antibodies from serum and other sample types. Antibody purification with Melon Gel may be performed with gravity-flow columns, spin columns or chromatography cartridges. Melon Gel IgG Purification saves time compared to classical Protein A or Protein G methods without compromising the quality of the antibody. Compared to antibody affinity purification with Protein A or Protein G, Melon Gel IgG Purification is simpler, faster and provides greater yields and higher purity for a wider variety of antibodies species.

Highlights:

• Simple, one-step protocol – IgG Purification with Melon Gel Resin does not require the binding, washing, and multiple elution steps required by Protein A and Protein G

• Rapid purification – antibody purification from serum is four to six times faster than Protein A or G methods

• High recovery and purity – antibody recovery from serum for many species is greater than 90% with greater than 80% purity

• Minimal dilution – reduced need to concentrate antibody before storage or use

• Robust purification – Melon Gel IgG Purification Kits work with a wide range of antibodies including many that do not purify well on Protein A or Protein G

• Gentle purification – no harsh antibody elution conditions helps retain IgG activity

• Reusable support – Melon Gel Resin can be used for multiple antibody purifications

• Available in various formats – spin columns, purification kits and chromatography cartridges

Specifications

• Quantity: 25 mL
• Description: Melon Gel IgG Purification Kit
• Format: Kit, Bottle
• Stationary Phase: Proprietary Ligand
• Column Type: Proprietary Resin, Affinity

• Product Line: Melon™
• Purification Target: IgG Antibodies
• Product Type: IgG Purification Kit
• Content And Storage: Sufficient For: IgG purification from 75 to 150 mL of serum (total approx. 2 g IgG from serum)
  • Melon Gel Support, 25 mL
  • Melon Gel Purification Buffer (dry mix to make 100X), 1 L
  • Melon Gel Regenerant (dry mix to make 1X), 1 L
  
  Store at 4°C