Thermo Scientific™ Mem-PER™ Eukaryotic Membrane Protein Extraction Kit

Description

Perform small-scale hydrophobic membrane-associated protein solubilization and enrichment with this simple reagent-based procedure.

Thermo Scientific™ Mem-PER Eukaryotic Membrane Protein Extraction Kit enables small-scale solubilization and enrichment of hydrophobic membrane-associated proteins in a simple reagent-based procedure.

Traditional methods for isolation of membrane protein are tedious and time-consuming, and they require gradient separation and expensive ultracentrifugation equipment. The Mem-PER Kit effectively isolates membrane proteins from cultured cells and tissues using a simple benchtop microcentrifuge procedure. Hydrophobic proteins having three or fewer membrane-spanning domains are effectively solubilized and isolated from hydrophilic (cytoplasmic) proteins using the three-reagent system. The kit makes possible the extraction and selective enrichment of integral and attached membrane proteins from cultured mammalian and yeast cells, as well as from hard and soft mammalian tissues.

Highlights:

• Extraction and isolation – produces minimal cross-contamination (typically <10%) of hydrophilic protein into the hydrophobic (membrane) protein fraction

• Cells or tissues – extracts protein from cultured mammalian and yeast cells and mammalian tissues

• Downstream compatibility – analyze membrane protein extracts by SDS-PAGE, Western blotting and protein assays

• Fast and simple – isolate membrane proteins in approximately one hour

• No special equipment required – requires only a benchtop microcentrifuge, tubes and pipetters

Includes:

Three buffers and detergent solutions necessary to make working reagents

Specifications

• Formulation: Three-component kit
• Description: Mem-PER Eukaryotic Membrane Protein Extraction Kit
• Includes: Mem-PER Reagent C, 40mL; Mem-PER Reagent B, 25mL; Mem-PER Reagent A, 10mL
• Target Proteins: Membrane
• Donor Source: Cultured Mammalian Cells, Cultured Yeast Cells, Animal Tissue
• Sufficient For: 60 to 80 samples each containing 5 million cells, 15mg yeast cell paste or 20mg tissue
• Quantity: 75 mL Kit

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov