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Gibco™ MesenPRO RS™ Medium
Description
MesenPRO RS™ Medium is a reduced serum (2%) medium specifically formulated to support the growth of human mesenchymal stem cells (MSCs) in culture. MesenPRO RS™ Medium consistently improves MSC expansion compared with conventional serum-supplemented media. Using MesenPRO RS™ Medium, MSCs can be expanded for multiple passages while maintaining their multipotential phenotype (i.e., differentiation into osteogenic, chondrogenic, and adipogenic lineages).
MesenPRO RS™ Medium consists of:- Basal liquid medium (MesenPRO RS™ Basal Medium)
- Frozen supplement (MesenPRO RS™ Growth Supplement), which is added at the time of use.
Formulated for the growth and expansion of mesenchymal stem cells.
Note: MesenPRO RS™ Medium has been developed for culturing MSCs at greater than clonal densities.
Recommended storage conditions:
- MesenPRO RS™ Basal Medium: Store in the dark at 2 to 8°C
- MesenPRO RS™ Growth Supplement: Store in the dark at -5 to -20°C
Both the Basal Medium and Growth Supplement are guaranteed stable for 24 months when properly stored.
Note: MesenPRO RS™ complete medium (basal, supplement and glutamine) is stable for 15 days when stored in the dark at 2 to 8°C. We do not recommend using the complete medium beyond 15 days.
Intended use(s):
in vitro diagnostic (IVD)
This reagent is classified as a medical device in several countries. If it is not regulated as a medical device in specific region, it should be used for laboratory use only.
Specifications
Specifications
| Cell Type | Mesenchymal Stem Cells |
| Classification | Serum-supplemented |
| Form | Liquid |
| Product Type | Stem Cell Media |
| Serum Level | Reduced Serum |
| Without Additives | No Glutamine, No Phenol Red |
| Product Line | MesenPRO |
| Quantity | 1 Kit |
| Species | Human |
| Content And Storage | MesenPRO RS™ Medium consists of: • 500 ml MesenPRO RS™ Basal Medium (store in the dark at 2°C to 8°C) • 10 ml MesenPRO RS™ Growth Supplement (store in the dark at -5°C to -20°C) The Basal Medium ships at room temperature, the Growth Supplement on dry ice. |
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Frequently Asked Questions (FAQs)
Yes, the MesenPRO RS Medium (with growth supplement added) can be used for both cell types.
MSCs may be cultured in either serum-containing medium or serum-free medium. The standard culturing conditions are DMEM, low glucose with 10% FBS. MSCs can also be grown in reduced-serum (2%) Gibco MesenPRO RS Medium (Cat. No. 12746-012), or in serum-free Gibco StemPro MSC SFM XenoFree Medium (Cat. No. A10675) and Gibco StemPro MSC SFM (Cat. No. A10332). In general, MSCs grow better under hypoxic conditions (2% O2).
Human MSCs can be identified by flow cytometry, typically displaying CD73+, CD90+, CD105+, CD11b-, and CD45- marker characteristics (Blood 109:4245 (2007)). Human MSC expresses Oct4, Sox2, and Rex-1; these may be verified using RT-PCR (Arthritis Res Ther 9:204 (2007)).
Mesenchymal stem cells (MSCs) are multipotent cells isolated primarily from bone marrow or fat tissues that exhibit the ability to differentiate into bone, cartilage, and fat cells. Under normal cell culture conditions, MSCs isolated from bone marrow are spindle shaped with the unique ability to adhere to uncoated plastic culture dishes (Arthritis Res Ther 9:204 (2007)).
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