Recombinant Protein;200UG;FITC-Labeled Human Mesothelin (296-580), Fc Tag (Cat. No. MSN-HF25x) is expressed from human HEK293 cells. It contains AA Glu 296 - Gly 580 (Accession # AAH09272.1). It is the FITC labeled form of Human Mesothelin (296-580), Fc Tag (Cat. No. MSN-H526x).;This protein carries a human IgG1 Fc tag at the N-terminus. The protein has a calculated MW of 59.1 kDa. The protein migrates as 60-67 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Anti-Human MSLN antibody at 10 μg/mL (100 μL/well) can bind FITC-Labeled Human Mesothelin (296-580), Fc Tag (Cat. No. MSN-HF25x) with a linear range of 0.039-0.625 μg/mL (QC tested).;293 cells were transfected with anti-MSLN-scFv and RFP tag. 2e5 of the cells were stained with B. FITC-Labeled Human Mesothelin (296-580), Fc Tag (Cat. No. MSN-HF25x, 0.3 µg/ml) and C. FITC-labeled protein control. A. Non-transfected 293 cells and C. FITC-labeled protein control were used as negative control. RFP was used