Recombinant Protein;FITC-Labeled Human Mesothelin / MSLN (296-580) Protein;200UG;FITC-Labeled Human Mesothelin (296-580) (Cat. No. MSN-HF223) is expressed from human HEK293 cells. It contains AA Glu 296 - Gly 580 (Accession # AAH09272). It is the FITC labeled form of Human Mesothelin (296-580) (Cat. No. MSN-H5223).;This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 33.0 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Anti-Human MSLN MAb at 10 μg/mL (100 μL/well) can bind FITC-Labeled Human Mesothelin (296-580) (Cat. No. MSN-HF223) with a linear range of 0.078-0.313 μg/mL (Ex.488 nm/Em.535 nm) (QC tested).;293 cells were transfected with anti-MSLN-scFv and RFP tag. 2e5 of the cells were stained with B. FITC-Labeled Human Mesothelin (296-580) (Cat. No. MSN-HF223, 1 μg/ml) and C. FITC-labeled protein control. A. Non-transfected 293 cells and C. FITC-labeled protein c