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ACROBiosystems Recombinant Protein;Biotinylated Human Mesothelin / MSLN (296-580), His Tag & Avi Tag (Avitag™);HEK293;200UG;MSN,MSLN,Mesothelin,MPF

Supplier:  ACROBiosystems MSNH82E9200UG

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Recombinant Protein;Biotinylated Human Mesothelin / MSLN (296-580), His Tag & Avi Tag (Avitag™);200UG;Biotinylated Human Mesothelin (296-580), His Tag, Avi Tag (MSN-H82E9) is expressed from human 293 cells (HEK293). It contains AA Glu 296 - Gly 580 (Accession # AAH09272.1).;This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 35.9 kDa. The protein migrates as 45-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Anti-Human MSLN Mab at 0.5 μg/mL (100 μL/well) can bind Biotinylated Human Mesothelin (296-580), His Tag, Avi Tag (Cat. No. MSN-H82E9) with a linear range of 0.1-0.78 ng/mL (QC tested).;293 cells were transfected with Anti-MSLN-scFv and RFP tag. 2e5 of the cells were first stained with B. Biotinylated Human Mesothelin (296-580), His Tag, Avi Tag (Cat. No. MSN-H82E9, 3 µg/ml) and C. Biotinylated Protein Control, followed by FITC Streptavidin. A. Non-transfected 293 cel

Catalog No. 50-165-8327


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