Abnova™ MET/CEN7 CISH Probe

Catalog No.	For Use With (Application)
89-335-551	CISH-P

Catalog No. 89-335-551 Supplier Abnova™ Supplier No. CG0010

Description

MET/CEN7 CISH Probe is designed for the qualitative detection of human MET gene and chromosome 7 alpha satellites in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).

The probe is intended to be used in combination with the CISH Implementation Kit 2, which provides necessary reagents for specimen pretreatment and post-hybridization processing.

Hybridization signals of digoxigenin-labeled polynucleotides appear dark green distinct dotdots (MET gene region), and dinitrophenyl-labeled polynucleotides appear bright red colored distinct dots (CEN 7).
Normal situation: In interphases of normal cells or cells without a amplification involving the MET gene locus, two green signals and two red signals appear.
Aberrant situation: In cells with amplification of the MET gene locus, an increased number of gene specific signals or signal clusters are visible. Other signal distribution may be observed in some abnormal samples which might result in a different signal pattern than described above, indicating variant rearrangements.
Unexpected signal patterns should be further investigated.

Specifications

• For Use With (Application): CISH-P
• Content And Storage: The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18 to 25°C) and mix briefly before use.